ArticleApplied and environmental microbiology1998
Genetic relatedness among environmental, clinical, and diseased-eel Vibrio vulnificus isolates from different geographic regions by ribotyping and randomly amplified polymorphic DNA PCR.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 51 citations in OpenAlex.
- Occurrence of clinical genotypeJournal of food science and technology · 2017Article
- Genome-wide SNP-genotyping array to study the evolution of the human pathogen Vibrio vulnificus biotype 3.PloS one · 2014Article
- Starvation induces phenotypic diversification and convergent evolution in Vibrio vulnificus.PloS one · 2014Article
- Genotypic Characterization of Vibrio vulnificus Clinical Isolates in Korea.Osong public health and research perspectives · 2011Article
- Evaluation of genotypic and phenotypic methods to distinguish clinical from environmental Vibrio vulnificus strains.Applied and environmental microbiology · 2009Article
- Emergence of a virulent clade of Vibrio vulnificus and correlation with the presence of a 33-kilobase genomic island.Applied and environmental microbiology · 2007Article
- Pulsed-field gel electrophoresis analysis of Vibrio vulnificus strains isolated from Taiwan and the United States.Applied and environmental microbiology · 2004Article
- Population genetics of Vibrio vulnificus: identification of two divisions and a distinct eel-pathogenic clone.Applied and environmental microbiology · 2003Article
- Intraspecific diversity of Vibrio vulnificus in Galveston Bay water and oysters as determined by randomly amplified polymorphic DNA PCR.Applied and environmental microbiology · 2003Article
- Sequence polymorphism of the 16S rRNA gene of Vibrio vulnificus is a possible indicator of strain virulence.Journal of clinical microbiology · 2003Article
- Random amplified polymorphic DNA analysis to differentiate strains of Vibrio vulnificus isolated from cockles and shrimps.The Malaysian journal of medical sciences : MJMS · 2000Article
- Differentiation of Vibrio alginolyticus strains isolated from Sardinian waters by ribotyping and a new rapid PCR fingerprinting method.Applied and environmental microbiology · 1999Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic relationships among 132 strains of Vibrio vulnificus (clinical, environmental, and diseased-eel isolates from different geographic origins, as well as seawater and shellfish isolates from the western Mediterranean coast, including reference strains) were analyzed by random amplified polymorphic DNA (RAPD) PCR. Results were validated by ribotyping. For ribotyping, DNAs were digested with KpnI and hybridized with an oligonucleotide probe complementary to a highly conserved sequence in the 23S rRNA gene. Random amplification of DNA was performed with M13 and T3 universal primers. The comparison between ribotyping and RAPD PCR revealed an overall agreement regarding the high level of homogeneity of diseased-eel isolates in contrast to the genetic heterogeneity of Mediterranean isolates. The latter suggests the existence of autochthonous clones present in Mediterranean coastal waters. Both techniques have revealed a genetic proximity among Spanish fish farm isolates and a close relationship between four Spanish eel farm isolates and some Mediterranean isolates. Whereas the differentiation within diseased-eel isolates was only possible by ribotyping, RAPD PCR was able to differentiate phenotypically atypical isolates of V. vulnificus. On the basis of our results, RAPD PCR is proposed as a better technique than ribotyping for rapid typing in the routine analysis of new V. vulnificus isolates.
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