ArticleApplied and environmental microbiology1998
Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Comparison of Immunomagnetic Bead Separation-Immunosensor Detection and Nested-PCR Methods for Detecting Mycobacterium avium Subspecies paratuberculosis in Cattle Feces.Journal of clinical laboratory analysis · 2025Article
- Application of the Actiphage® Assay to Detect ViableFrontiers in veterinary science · 2021Article
- Efficiency of DNA Isolation Methods Based on Silica Columns and Magnetic Separation Tested for the Detection ofMaterials (Basel, Switzerland) · 2020Article
- Article
- Magnetic Separation Methods for the Detection ofBioMed research international · 2017Review
- Article
- Evaluation of immunomagnetic separation for the detection of Salmonella in surface waters by polymerase chain reaction.International journal of environmental research and public health · 2014Article
- Whole-genome sequences of Chlamydia trachomatis directly from clinical samples without culture.Genome research · 2013Article
- Production and evaluation of antibodies and phage display-derived peptide ligands for immunomagnetic separation of Mycobacterium bovis.Journal of clinical microbiology · 2012Article
- Separation of the bacterial species, Escherichia coli, from mixed-species microbial communities for transcriptome analysis.BMC microbiology · 2011Article
- Maximizing capture efficiency and specificity of magnetic separation for Mycobacterium avium subsp. paratuberculosis cells.Applied and environmental microbiology · 2010Article
- Bacterial capture by peptide-mimetic oligoacyllysine surfaces.Applied and environmental microbiology · 2010Article
- Development of an immunocapture-polymerase chain reaction assay using IgY to detect Mycobacterium avium subsp. paratuberculosis.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2010Article
- Production of and applications for a polyclonal IgY diagnostic reagent specific for Mycobacterium avium subsp. paratuberculosis.Journal of microbiology (Seoul, Korea) · 2009Article
- Optimization of a phage amplification assay to permit accurate enumeration of viable Mycobacterium avium subsp. paratuberculosis cells.Applied and environmental microbiology · 2009Article
- Immunomagnetic separation and coagglutination of Vibrio parahaemolyticus with anti-flagellar protein monoclonal antibody.Clinical and vaccine immunology : CVI · 2008Article
- Risk factors for the introduction and within-herd transmission of Mycobacterium avium subspecies paratuberculosis (MAP) infection on 59 Irish dairy herds.Irish veterinary journal · 2008Article
- Isolation of high-affinity single-chain antibodies against Mycobacterium avium subsp. paratuberculosis surface proteins from sheep with Johne's disease.Clinical and vaccine immunology : CVI · 2006Article
- Peptide aMptD-mediated capture PCR for detection of Mycobacterium avium subsp. paratuberculosis in bulk milk samples.Applied and environmental microbiology · 2006Article
- Comparing recovering efficiency of immunomagnetic separation and centrifugation of mycobacteria in metalworking fluids.Journal of industrial microbiology & biotechnology · 2005Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An immunomagnetic separation (IMS) technique was developed to facilitate selective isolation of Mycobacterium paratuberculosis cells from milk. Rabbit polyclonal antibodies against radiation-killed intact M. paratuberculosis cells were produced and used to coat sheep anti-rabbit immunoglobulin G (IgG) type M-280 Dynabeads. The rabbit anti-M. paratuberculosis IgG-coated beads (IMB) reacted strongly with laboratory strains of M. paratuberculosis as determined by slide agglutination, and microscopic examination confirmed that M. paratuberculosis cells attached to the IMB. The IMB were found to have a maximum binding capacity of 10(4) to 10(5) CFU of M. paratuberculosis. Studies showed that IMS selectively recovered M. paratuberculosis from inoculated milk containing as few as 10 CFU of M. paratuberculosis per ml when 10 microliter IMB (ca. 10(6) beads) was added to 1 ml of milk and the preparation was incubated for 30 min at room temperature with gentle agitation. Larger volumes of milk (10 and 50 ml) were centrifuged and resuspended in 1 ml of phosphate-buffered saline-0.05% Tween 20 prior to IMS in order to increase the sensitivity of the method. Currently, primary isolation of M. paratuberculosis from a milk sample relies on chemical decontamination, followed by culturing on Herrold's egg yolk medium, which must be incubated at 37 degreesC for up to 18 weeks. The potential value of our IMS method is as an aid for rapid detection of M. paratuberculosis in milk when it is used in conjunction with end point detection methods, such as IS900 PCR or an enzyme-linked immunosorbent assay.
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