ArticleMolecular biology of the cell1998
Tim23p contains separate and distinct signals for targeting to mitochondria and insertion into the inner membrane.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 67 citations in OpenAlex.
- Article
- Biogenesis of Mitochondrial Metabolite Carriers.Biomolecules · 2020Review
- The mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segments.BMC biology · 2020Article
- Cardiolipin mediates membrane and channel interactions of the mitochondrial TIM23 protein import complex receptor Tim50.Science advances · 2017Article
- Role of Tim17 Transmembrane Regions in Regulating the Architecture of Presequence Translocase and Mitochondrial DNA Stability.Molecular and cellular biology · 2017Article
- Targeting of a polytopic membrane protein to the inner envelope membrane of chloroplasts in vivo involves multiple transmembrane segments.Journal of experimental botany · 2014Article
- Molecular insights revealing interaction of Tim23 and channel subunits of presequence translocase.Molecular and cellular biology · 2013Article
- Mitochondria of the yeasts Saccharomyces cerevisiae and Kluyveromyces lactis contain nuclear rDNA-encoded proteins.PloS one · 2011Article
- Quaternary structure of the mitochondrial TIM23 complex reveals dynamic association between Tim23p and other subunits.Molecular biology of the cell · 2008Article
- The Tim9p/10p and Tim8p/13p complexes bind to specific sites on Tim23p during mitochondrial protein import.Molecular biology of the cell · 2007Article
- The WD-repeats of Net2p interact with Dnm1p and Fis1p to regulate division of mitochondria.Molecular biology of the cell · 2003Article
- The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins.The Journal of cell biology · 2002Article
- Membrane potential-driven protein import into mitochondria. The sorting sequence of cytochrome b(2) modulates the deltapsi-dependence of translocation of the matrix-targeting sequence.Molecular biology of the cell · 2000Article
- Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria.The Journal of cell biology · 2000Article
- Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane.Molecular and cellular biology · 2000Article
- Signals and receptors--the translocation machinery on the mitochondrial surface.Journal of bioenergetics and biomembranes · 2000Review
- Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane.Molecular biology of the cell · 2000Article
- Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Tim23 protein is an essential inner membrane (IM) component of the yeast mitochondrial protein import pathway. Tim23p does not carry an amino-terminal presequence; therefore, the targeting information resides within the mature protein. Tim23p is anchored in the IM via four transmembrane segments and has two positively charged loops facing the matrix. To identify the import signal for Tim23p, we have constructed several altered versions of the Tim23 protein and examined their function and import in yeast cells, as well as their import into isolated mitochondria. We replaced the positively charged amino acids in one or both loops with alanine residues and found that the positive charges are not required for import into mitochondria, but at least one positively charged loop is required for insertion into the IM. Furthermore, we find that the signal to target Tim23p to mitochondria is carried in at least two of the hydrophobic transmembrane segments. Our results suggest that Tim23p contains separate import signals: hydrophobic segments for targeting Tim23p to mitochondria, and positively charged loops for insertion into the IM. We therefore propose that Tim23p is imported into mitochondria in at least two distinct steps.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.