ArticleMolecular biology of the cell1998
Organization of highly acetylated chromatin around sites of heterogeneous nuclear RNA accumulation.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 94 citations in OpenAlex.
- Nuclear speckle-associated perispeckle networks partition active chromatin into gene expression niches.Cell reports · 2026Article
- Nuclear Compartments: An Incomplete Primer to Nuclear Compartments, Bodies, and Genome Organization Relative to Nuclear Architecture.Cold Spring Harbor perspectives in biology · 2022Review
- The solid and liquid states of chromatin.Epigenetics & chromatin · 2021Review
- Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler.The Journal of cell biology · 2018Article
- Sos1 disruption impairs cellular proliferation and viability through an increase in mitochondrial oxidative stress in primary MEFs.Oncogene · 2016Article
- Correlative fluorescence and EFTEM imaging of the organized components of the mammalian nucleus.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Nonlinear optical imaging and Raman microspectrometry of the cell nucleus throughout the cell cycle.Biophysical journal · 2010Article
- Cajal's contribution to the knowledge of the neuronal cell nucleus.Chromosoma · 2009Review
- Elucidating chromatin and nuclear domain architecture with electron spectroscopic imaging.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Review
- The transcriptional regulator CBP has defined spatial associations within interphase nuclei.PLoS computational biology · 2006Article
- Quantitative analysis of CBP- and P300-induced histone acetylations in vivo using native chromatin.Molecular and cellular biology · 2003Article
- Clustering of multiple specific genes and gene-rich R-bands around SC-35 domains: evidence for local euchromatic neighborhoods.The Journal of cell biology · 2003Article
- FBI-1 can stimulate HIV-1 Tat activity and is targeted to a novel subnuclear domain that includes the Tat-P-TEFb-containing nuclear speckles.Molecular biology of the cell · 2002Article
- Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo.Molecular and cellular biology · 2001Article
- The cells of the rabbit meniscus: their arrangement, interrelationship, morphological variations and cytoarchitecture.Journal of anatomy · 2001Article
- The transcription coactivator CBP is a dynamic component of the promyelocytic leukemia nuclear body.The Journal of cell biology · 2001Article
- Article
- Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments.The Journal of cell biology · 2000Article
- Promyelocytic leukemia (PML) nuclear bodies are protein structures that do not accumulate RNA.The Journal of cell biology · 2000Article
- Spatial relationship between transcription sites and chromosome territories.The Journal of cell biology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histones found within transcriptionally competent and active regions of the genome are highly acetylated. Moreover, these highly acetylated histones have very short half-lives. Thus, both histone acetyltransferases and histone deacetylases must enrich within or near these euchromatic regions of the interphase chromatids. Using an antibody specific for highly acetylated histone H3, we have investigated the organization of transcriptionally active and competent chromatin as well as nuclear histone acetyltransferase and deacetylase activities. We observe an exclusion of highly acetylated chromatin around the periphery of the nucleus and an enrichment near interchromatin granule clusters (IGCs). The highly acetylated chromatin is found in foci that may reflect the organization of highly acetylated chromatin into "chromonema" fibers. Transmission electron microscopy of Indian muntjac fibroblast cell nuclei indicates that the chromatin associated with the periphery of IGCs remains relatively condensed, most commonly found in domains containing chromatin folded beyond 30 nm. Using electron spectroscopic imaging, we demonstrate that IGCs are clusters of ribonucleoprotein particles. The individual granules comprise RNA-rich fibrils or globular regions that fold into individual granules. Quantitative analysis of individual granules indicates that they contain variable amounts of RNA estimated between 1.5 and >10 kb. We propose that interchromatin granules are heterogeneous nuclear RNA-containing particles, some of which may be pre-mRNA generated by nearby transcribed chromatin. An intermediary zone between the IGC and surrounding chromatin is described that contains factors with the potential to provide specificity to the localization of sequences near IGCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.