ArticleMolecular biology of the cell1998
Real-time imaging of the axonal transport of granules containing a tissue plasminogen activator/green fluorescent protein hybrid.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 84 citations in OpenAlex.
- Going Too Far Is the Same as Falling ShortFrontiers in cellular neuroscience · 2019Review
- Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA).Frontiers in cellular neuroscience · 2019Article
- Advances in TRH signaling.Reviews in endocrine & metabolic disorders · 2016Review
- The neurotrophin receptor p75 mediates gp120-induced loss of synaptic spines in aging mice.Neurobiology of aging · 2016Article
- Tissue Plasminogen Activator Expression Is Restricted to Subsets of Excitatory Pyramidal Glutamatergic Neurons.Molecular neurobiology · 2016Article
- Stochastic Subcellular Organization of Dense-Core Vesicles Revealed by Point Pattern Analysis.Biophysical journal · 2016Article
- The Role of Proteases in Hippocampal Synaptic Plasticity: Putting Together Small Pieces of a Complex Puzzle.Neurochemical research · 2016Review
- Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles.Molecular biology of the cell · 2015Article
- Physiological and pathological roles of tissue plasminogen activator and its inhibitor neuroserpin in the nervous system.Frontiers in cellular neuroscience · 2015Review
- Hindered submicron mobility and long-term storage of presynaptic dense-core granules revealed by single-particle tracking.Developmental neurobiology · 2012Article
- Transcription factor plasmid binding modulates microtubule interactions and intracellular trafficking during gene transfer.Gene therapy · 2012Article
- The plasminogen activation system and the regulation of catecholaminergic function.Journal of biomedicine & biotechnology · 2012Review
- Automated image analysis for tracking cargo transport in axons.Microscopy research and technique · 2011Article
- Syntaxin clusters assemble reversibly at sites of secretory granules in live cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Article
- Efficient copackaging and cotransport yields postsynaptic colocalization of neuromodulators associated with synaptic plasticity.Developmental neurobiology · 2008Article
- Developmental profile of tissue plasminogen activator in postnatal Long Evans rat visual cortex.Molecular vision · 2008Article
- Not all secretory granules are created equal: Partitioning of soluble content proteins.Molecular biology of the cell · 2006Article
- Article
- Mechanisms of transport and exocytosis of dense-core granules containing tissue plasminogen activator in developing hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A hybrid protein, tPA/GFP, consisting of rat tissue plasminogen activator (tPA) and green fluorescent protein (GFP) was expressed in PC12 cells and used to study the distribution, secretory behavior, and dynamics of secretory granules containing tPA in living cells with a neuronal phenotype. High-resolution images demonstrate that tPA/GFP has a growth cone-biased distribution in differentiated cells and that tPA/GFP is transported in granules of the regulated secretory pathway that colocalize with granules containing secretogranin II. Time-lapse images of secretion reveal that secretagogues induce substantial loss of cellular tPA/GFP fluorescence, most importantly from growth cones. Time-lapse images of the axonal transport of granules containing tPA/GFP reveal a surprising complexity to granule dynamics. Some granules undergo canonical fast axonal transport; others move somewhat more slowly, especially in highly fluorescent neurites. Most strikingly, granules traffic bidirectionally along neurites to an extent that depends on granule accumulation, and individual granules can reverse their direction of motion. The retrograde component of this bidirectional transport may help to maintain cellular homeostasis by transporting excess tPA/GFP back toward the cell body. The results presented here provide a novel view of the axonal transport of secretory granules. In addition, the results suggest that tPA is targeted for regulated secretion from growth cones of differentiated cells, strategically positioning tPA to degrade extracellular barriers or to activate other barrier-degrading proteases during axonal elongation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.