ArticleMolecular biology of the cell1998
An alpha-tubulin mutant destabilizes the heterodimer: phenotypic consequences and interactions with tubulin-binding proteins.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Benzimidazole resistance-associated mutations improve theVeterinary world · 2024Article
- The novel microtubule-associated CAP-glycine protein Cgp1 governs growth, differentiation, and virulence of Cryptococcus neoformans.Virulence · 2018Article
- Microtubule disruption stimulates P-body formation.RNA (New York, N.Y.) · 2007Article
- Mutations in alpha-tubulin cause abnormal neuronal migration in mice and lissencephaly in humans.Cell · 2007Article
- A novel step in beta-tubulin folding is important for heterodimer formation in Saccharomyces cerevisiae.Genetics · 2003Article
- Dissociation of the tubulin dimer is extremely slow, thermodynamically very unfavorable, and reversible in the absence of an energy source.Molecular biology of the cell · 2002Article
- Protection from free beta-tubulin by the beta-tubulin binding protein Rbl2p.Molecular and cellular biology · 2002Article
- Single site alpha-tubulin mutation affects astral microtubules and nuclear positioning during anaphase in Saccharomyces cerevisiae: possible role for palmitoylation of alpha-tubulin.Molecular biology of the cell · 2001Article
- Stathmin/Op18 phosphorylation is regulated by microtubule assembly.Molecular biology of the cell · 2001Article
- The cofactor-dependent pathways for alpha- and beta-tubulins in microtubule biogenesis are functionally different in fission yeast.Genetics · 2000Article
- Function of tubulin binding proteins in vivo.Genetics · 2000Article
- Structure-function relationships in yeast tubulins.Molecular biology of the cell · 2000Article
- Functional dissection and hierarchy of tubulin-folding cofactor homologues in fission yeast.Molecular biology of the cell · 1999Article
- Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin.The Journal of cell biology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many effectors of microtubule assembly in vitro enhance the polymerization of subunits. However, several Saccharomyces cerevisiae genes that affect cellular microtubule-dependent processes appear to act at other steps in assembly and to affect polymerization only indirectly. Here we use a mutant alpha-tubulin to probe cellular regulation of microtubule assembly. tub1-724 mutant cells arrest at low temperature with no assembled microtubules. The results of several assays reported here demonstrate that the heterodimer formed between Tub1-724p and beta-tubulin is less stable than wild-type heterodimer. The unstable heterodimer explains several conditional phenotypes conferred by the mutation. These include the lethality of tub1-724 haploid cells when the beta-tubulin-binding protein Rbl2p is either overexpressed or absent. It also explains why the TUB1/tub1-724 heterozygotes are cold sensitive for growth and why overexpression of Rbl2p rescues that conditional lethality. Both haploid and heterozygous tub1-724 cells are inviable when another microtubule effector, PAC2, is overexpressed. These effects are explained by the ability of Pac2p to bind alpha-tubulin, a complex we demonstrate directly. The results suggest that tubulin-binding proteins can participate in equilibria between the heterodimer and its components.
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