ArticleProceedings of the National Academy of Sciences of the United States of America1998
A single gene of chloroplast origin codes for mitochondrial and chloroplastic methionyl-tRNA synthetase in Arabidopsis thaliana.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 85 citations in OpenAlex.
- Organelle genomes reveal adaptive evolution and phylogenetic position of the endangeredFrontiers in plant science · 2025Article
- The Poplar Rust-Induced Secreted Protein (RISP) Inhibits the Growth of the Leaf Rust Pathogen Melampsora larici-populina and Triggers Cell Culture Alkalinisation.Frontiers in plant science · 2016Article
- EMBRYONIC FACTOR 31 encodes a tyrosyl-tRNA synthetase that is essential for seed development.Molecular biology reports · 2012Article
- Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots.Proteome science · 2012Article
- Atypical thioredoxins in poplar: the glutathione-dependent thioredoxin-like 2.1 supports the activity of target enzymes possessing a single redox active cysteine.Plant physiology · 2012Article
- Transfection of plant mitochondria and in organello gene integration.Nucleic acids research · 2011Article
- Plant mitochondria possess a short-patch base excision DNA repair pathway.Nucleic acids research · 2009Article
- Conservation of dual-targeted proteins in Arabidopsis and rice points to a similar pattern of gene-family evolution.Molecular genetics and genomics : MGG · 2009Article
- Import of tRNAs and aminoacyl-tRNA synthetases into mitochondria.Current genetics · 2009Review
- Biochemical and genomic characterization of terpene synthases in Magnolia grandiflora.Plant physiology · 2008Article
- Plant glutathione peroxidases are functional peroxiredoxins distributed in several subcellular compartments and regulated during biotic and abiotic stresses.Plant physiology · 2006Article
- The plant-specific ssDNA binding protein OSB1 is involved in the stoichiometric transmission of mitochondrial DNA in Arabidopsis.The Plant cell · 2006Article
- Overexpression of farnesyl diphosphate synthase in Arabidopsis mitochondria triggers light-dependent lesion formation and alters cytokinin homeostasis.Plant molecular biology · 2006Article
- Nuclear photosynthetic gene expression is synergistically modulated by rates of protein synthesis in chloroplasts and mitochondria.The Plant cell · 2006Article
- Dual targeting is the rule for organellar aminoacyl-tRNA synthetases in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Poplar peroxiredoxin Q. A thioredoxin-linked chloroplast antioxidant functional in pathogen defense.Plant physiology · 2004Article
- Article
- Plant mitochondria actively import DNA via the permeability transition pore complex.The EMBO journal · 2003Article
- Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family.Plant physiology · 2002Article
- Mitochondrial targeting and membrane anchoring of a viral replicase in plant and yeast cells.Journal of virology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One-fifth of the tRNAs used in plant mitochondrial translation is coded for by chloroplast-derived tRNA genes. To understand how aminoacyl-tRNA synthetases have adapted to the presence of these tRNAs in mitochondria, we have cloned an Arabidopsis thaliana cDNA coding for a methionyl-tRNA synthetase. This enzyme was chosen because chloroplast-like elongator tRNAMet genes have been described in several plant species, including A. thaliana. We demonstrate here that the isolated cDNA codes for both the chloroplastic and the mitochondrial methionyl-tRNA synthetase (MetRS). The protein is transported into isolated chloroplasts and mitochondria and is processed to its mature form in both organelles. Transient expression assays using the green fluorescent protein demonstrated that the N-terminal region of the MetRS is sufficient to address the protein to both chloroplasts and mitochondria. Moreover, characterization of MetRS activities from mitochondria and chloroplasts of pea showed that only one MetRS activity exists in each organelle and that both are indistinguishable by their behavior on ion exchange and hydrophobic chromatographies. The high degree of sequence similarity between A. thaliana and Synechocystis MetRS strongly suggests that the A. thaliana MetRS gene described here is of chloroplast origin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.