ArticleProceedings of the National Academy of Sciences of the United States of America1998
Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 122 citations in OpenAlex.
- Ultralong-Time Recovery and Low-Voltage Electroporation for Biological Cell Monitoring Enabled by a Microsized Multipulse Framework.ACS omega · 2021Article
- Micromotor-based localized electroporation and gene transfection of mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Active particles as mobile microelectrodes for selective bacteria electroporation and transport.Science advances · 2020Article
- Current Trends of Microfluidic Single-Cell Technologies.International journal of molecular sciences · 2018Review
- Optimization of single-cell electroporation protocol for forced gene expression in primary neuronal cultures.Molecular biotechnology · 2014Article
- Single-cell juxtacellular transfection and recording technique.Pflugers Archiv : European journal of physiology · 2013Article
- Redox-filled Carbon-Fiber Microelectrodes for Single-Cell Exocytosis.Electroanalysis · 2013Article
- Nanofountain probe electroporation (NFP-E) of single cells.Nano letters · 2013Article
- Ultra-localized single cell electroporation using silicon nanowires.Lab on a chip · 2013Article
- Micro-/nanofluidics based cell electroporation.Biomicrofluidics · 2013Article
- Single-cell electroporation using a multifunctional pipette.Lab on a chip · 2012Article
- Long-term gene-silencing effects of siRNA introduced by single-cell electroporation into postmitotic CNS neurons.Neurochemical research · 2011Article
- Generation of phospholipid vesicle-nanotube networks and transport of molecules therein.Nature protocols · 2011Article
- Mechanisms for the intracellular manipulation of organelles by conventional electroporation.Biophysical journal · 2010Article
- Control of the release of freely diffusing molecules in single-cell electroporation.Analytical chemistry · 2009Article
- Single cell and neural process experimentation using laterally applied electrical fields between pairs of closely apposed microelectrodes with vertical sidewalls.Biosensors & bioelectronics · 2009Article
- Parallel single-cell light-induced electroporation and dielectrophoretic manipulation.Lab on a chip · 2009Article
- Single-cell transfection by electroporation using an electrolyte/plasmid-filled capillary.Analytical chemistry · 2009Article
- Lab-on-a-chip technologies for proteomic analysis from isolated cells.Journal of the Royal Society, Interface · 2008Article
- Finite element analysis of microelectrotension of cell membranes.Biomechanics and modeling in mechanobiology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe an efficient technique for the selective chemical and biological manipulation of the contents of individual cells. This technique is based on the electric-field-induced permeabilization (electroporation) in biological membranes using a low-voltage pulse generator and microelectrodes. A spatially highly focused electric field allows introduction of polar cell-impermeant solutes such as fluorescent dyes, fluorogenic reagents, and DNA into single cells. The high spatial resolution of the technique allows for design of, for example, cellular network constructions in which cells in close contact with each other can be made to possess different biochemical, biophysical, and morphological properties. Fluorescein, and fluo-3 (a calcium-sensitive fluorophore), are electroporated into the soma of cultured single progenitor cells derived from adult rat hippocampus. Fluo-3 also is introduced into individual submicrometer diameter processes of thapsigargin-treated progenitor cells, and a plasmid vector cDNA construct (pRAY 1), expressing the green fluorescent protein, is electroporated into cultured single COS 7 cells. At high electric field strengths, observations of dye-transfer into organelles are proposed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.