ArticleThe Plant cell1998
An intragenic suppressor of the Arabidopsis floral organ identity mutant apetala3-1 functions by suppressing defects in splicing.
Article in The Plant cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- From the archives: Boosting rice immunity, phosophoglucose isomerases influencing seed yield, and regulation of mRNA splicing.The Plant cell · 2023Article
- Article
- A High Temperature-Dependent Mitochondrial Lipase EXTRA GLUME1 Promotes Floral Phenotypic Robustness against Temperature Fluctuation in Rice (Oryza sativa L.).PLoS genetics · 2016Article
- Comparative expression analysis of senescence gene CsNAP and B-class floral development gene CsAP3 during different stages of flower development in Saffron (Crocus sativus L.).Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
- Alternative splicing at the intersection of biological timing, development, and stress responses.The Plant cell · 2013Review
- A single-base substitution suppresses flower color mutation caused by a novel miniature inverted-repeat transposable element in gentian.Molecular genetics and genomics : MGG · 2011Article
- The glabra1 mutation affects cuticle formation and plant responses to microbes.Plant physiology · 2010Article
- Analyses of the floral organ morphogenesis and the differentially expressed genes of an apetalous flower mutant in Brassica napus.Plant cell reports · 2008Article
- The homeotic protein AGAMOUS controls late stamen development by regulating a jasmonate biosynthetic gene in Arabidopsis.The Plant cell · 2007Article
- DORNROSCHEN-LIKE, an AP2 gene, is necessary for stamen emergence in Arabidopsis.Plant molecular biology · 2007Article
- Complete reannotation of the Arabidopsis genome: methods, tools, protocols and the final release.BMC biology · 2005Article
- Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2 of Arabidopsis.Plant physiology · 2002Article
- Heritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Mutational analysis of a plant branchpoint and polypyrimidine tract required for constitutive splicing of a mini-exon.RNA (New York, N.Y.) · 2002Article
- RBP45 and RBP47, two oligouridylate-specific hnRNP-like proteins interacting with poly(A)+ RNA in nuclei of plant cells.RNA (New York, N.Y.) · 2000Article
- The AG dinucleotide terminating introns is important but not always required for pre-mRNA splicing in the maize endospermPlant physiology · 1999Article
- Temperature-sensitive splicing in the floral homeotic mutant apetala3-1.The Plant cell · 1998Article
Corrections and comments
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis floral organ identity gene APETALA3 (AP3) specifies the identity of petals and stamens in the flower. In flowers mutant for the temperature-sensitive ap3-1 allele, the petals and stamens are partially converted to sepals and carpels, respectively. ap3-1 contains a single nucleotide change in the AP3 gene that alters both an amino acid in the AP3 protein and the 5' splice consensus site for intron 5. Surprisingly, the Ap3-1 mutant phenotype is not due to the missense mutation but instead is due to defects in splicing; specifically, exon 5 is frequently skipped by the splicing machinery at the restrictive temperature. In a screen for suppressors of ap3-1, we isolated an intragenic suppressor, ap3-11, that functions to suppress the splicing defects of ap3-1. Using a reverse transcriptase-polymerase chain reaction assay, we demonstrate that the percentage of full-length exon 5-containing AP3 RNAs correlates with the phenotype of the flowers in both ap3-1 and ap3-11. Rather surprisingly, the ap3-11 suppressor mutation is located in intron 4. One model explaining the function of ap3-11 is that the ap3-11 suppressor creates a novel branch point sequence that causes exon 5 to be more frequently recognized by the splicing machinery. The identification of such a suppressor strongly suggests that exon-scanning models of intron-exon recognition are operative in plants.
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