ArticleThe EMBO journal1998
Chromatin immunoselection defines a TAL-1 target gene.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 55 citations in OpenAlex.
- The Hematopoietic Stem and Progenitor Cell Cistrome: GATA Factor-Dependent cis-Regulatory Mechanisms.Current topics in developmental biology · 2016Review
- Article
- A 3-bp deletion in the HBS1L-MYB intergenic region on chromosome 6q23 is associated with HbF expression.Blood · 2011Article
- Control of the differentiation of regulatory T cells and T(H)17 cells by the DNA-binding inhibitor Id3.Nature immunology · 2011Article
- Networking erythropoiesis.The Journal of experimental medicine · 2010Review
- Molecular hallmarks of endogenous chromatin complexes containing master regulators of hematopoiesis.Molecular and cellular biology · 2008Article
- A computational model of quantitative chromatin immunoprecipitation (ChIP) analysis.Cancer informatics · 2008Article
- A positive role for NLI/Ldb1 in long-range beta-globin locus control region function.Molecular cell · 2007Article
- TAL-1/SCL and its partners E47 and LMO2 up-regulate VE-cadherin expression in endothelial cells.Molecular and cellular biology · 2007Article
- ETO2 coordinates cellular proliferation and differentiation during erythropoiesis.The EMBO journal · 2006Article
- GATA1 function, a paradigm for transcription factors in hematopoiesis.Molecular and cellular biology · 2005Article
- A novel E2 box-GATA element modulates Cdc6 transcription during human cells polyploidization.Nucleic acids research · 2004Article
- Identification of a TAL1 target gene reveals a positive role for the LIM domain-binding protein Ldb1 in erythroid gene expression and differentiation.Molecular and cellular biology · 2003Article
- A major role for the TATA box in recruitment of chromatin modifying complexes to a globin gene promoter.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Gradient of E2A activity in B-cell development.Molecular and cellular biology · 2002Article
- Use of chromatin immunoprecipitation to clone novel E2F target promoters.Molecular and cellular biology · 2001Article
- The DNA binding activity of TAL-1 is not required to induce leukemia/lymphoma in mice.Oncogene · 2001Article
- Acetylation of a specific promoter nucleosome accompanies activation of the epsilon-globin gene by beta-globin locus control region HS2.Molecular and cellular biology · 2001Article
- A pentamer transcriptional complex including tal-1 and retinoblastoma protein downmodulates c-kit expression in normal erythroblasts.Molecular and cellular biology · 2000Article
- mSin3A regulates murine erythroleukemia cell differentiation through association with the TAL1 (or SCL) transcription factor.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the major functions of the basic helix-loop-helix transcription factor TAL-1 in hematopoiesis and T-cell leukemogenesis, no TAL-1 target gene has been identified. Using immunoprecipitation of genomic fragments bound to TAL-1 in the chromatin of murine erythro-leukemia (MEL) cells, we found that 10% of the immunoselected fragments contained a CAGATG or a CAGGTG E-box, followed by a GATA site. We studied one of these fragments containing two E-boxes, CAGATG and CAGGTC, followed by a GATA motif, and showed that TAL-1 binds to the CAGGTG E-box with an affinity modulated by the CAGATG or the GATA site, and that the CAGGTG-GATA motif exhibits positive transcriptional activity in MEL but not in HeLa cells. This immunoselected sequence is located within an intron of a new gene co-expressed with TAL-1 in endothelial and erythroid cells, but not expressed in fibroblasts or adult liver where no TAL-1 mRNA was detected. Finally, in vitro differentiation of embryonic stem cells towards the erythro/megakaryocytic pathways showed that the TAL-1 target gene expression followed TAL-1 and GATA-1 expression. These results establish that TAL-1 is likely to activate its target genes through a complex that binds an E-box-GATA motif and define the first gene regulated by TAL-1.
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