ArticleNucleic acids research1998
Conserved DNA sequences adjacent to chromosome fragmentation and telomere addition sites in Euplotes crassus.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- The mechanistic and evolutionary diversity of programmed DNA elimination.Nature reviews. Genetics · 2026Review
- A sequence motif for DNA double-strand break and telomere healing during programmed DNA elimination.bioRxiv : the preprint server for biology · 2026Article
- Soma-derived 30-nt small RNAs are coupled with chromosome breakage and precisely target nontransposon DNA against elimination inScience advances · 2025Article
- Functions and mechanisms of eukaryotic RNA-guided programmed DNA elimination.Biochemical Society transactions · 2025Review
- End resection and telomere healing of DNA double-strand breaks during nematode programmed DNA elimination.Nucleic acids research · 2024Article
- End resection and telomere healing of DNA double-strand breaks during nematode programmed DNA elimination.bioRxiv : the preprint server for biology · 2024Article
- From germline genome to highly fragmented somatic genome: genome-wide DNA rearrangement during the sexual process in ciliated protists.Marine life science & technology · 2024Article
- Programmed chromosome fragmentation in ciliated protozoa: multiple means to chromosome ends.Microbiology and molecular biology reviews : MMBR · 2023Review
- The macronuclear genome of the Antarctic psychrophilic marine ciliate Euplotes focardii reveals new insights on molecular cold adaptation.Scientific reports · 2021Article
- Biodiversity-based development and evolution: the emerging research systems in model and non-model organisms.Science China. Life sciences · 2021Review
- Programmed genome rearrangements in ciliates.Cellular and molecular life sciences : CMLS · 2020Review
- Genome analyses of the new model protist Euplotes vannus focusing on genome rearrangement and resistance to environmental stressors.Molecular ecology resources · 2019Article
- Twisted Tales: Insights into Genome Diversity of Ciliates Using Single-Cell 'Omics.Genome biology and evolution · 2018Article
- Chromosome fusions triggered by noncoding RNA.RNA biology · 2017Article
- Programmed Genome Rearrangements in the Ciliate Oxytricha.Microbiology spectrum · 2014Review
- Characterization and expression of the gene encoding En-MAPK1, an intestinal cell kinase (ICK)-like kinase activated by the autocrine pheromone-signaling loop in the Polar Ciliate, Euplotes nobilii.International journal of molecular sciences · 2013Article
- Intron Evolution and Information processing in the DNA polymerase alpha gene in spirotrichous ciliates: a hypothesis for interconversion between DNA and RNA deletion.Biology direct · 2007Article
- Conserved linkage of two genes on the same macronuclear chromosome in spirotrichous ciliates.Chromosoma · 2006Article
- Developmentally regulated chromosome fragmentation linked to imprecise elimination of repeated sequences in paramecia.Eukaryotic cell · 2003Article
- Tec3, a new developmentally eliminated DNA element in Euplotes crassus.Eukaryotic cell · 2003Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
During the formation of a new macronucleus in the ciliate Euplotes crassus, micronuclear chromosomes are reproducibly broken at approximately 10 000 sites. This chromosome fragmentation process is tightly coupled with de novo telomere synthesis by the telomerase ribonucleoprotein complex, generating short linear macronuclear DNA molecules. In this study, the sequences of 58 macronuclear DNA termini and eight regions of the micronuclear genome containing chromosome fragmentation/telomere addition sites were determined. Through a statistically based analysis of these data, along with previously published sequences, we have defined a 10 bp conserved sequence element (E-Cbs, 5'-HATTGAAaHH-3', H = A, C or T) near chromosome fragmentation sites. The E-Cbs typically resides within the DNA destined to form a macronuclear DNA molecule, but can also reside within flanking micronuclear DNA that is eliminated during macronuclear development. The location of the E-Cbs in macronuclear-destined versus flanking micronuclear DNA leads us to propose a model of chromosome fragmentation that involves a 6 bp staggered cut in the chromosome. The identification of adjacent macronuclear-destined sequences that overlap by 6 bp provides support for the model. Finally, our data provide evidence that telomerase is able to differentiate between newly generated ends that contain partial telomeric repeats and those that do not in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.