ArticleThe Journal of cell biology1998
Disruption of the talin gene compromises focal adhesion assembly in undifferentiated but not differentiated embryonic stem cells.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 177 citations in OpenAlex.
- TLN1: an oncogene associated with tumorigenesis and progression.Discover oncology · 2024Review
- Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis.Science advances · 2024Article
- Dietary Polyphenols Effects on Focal Adhesion Plaques and Metalloproteinases in Cancer Invasiveness.Biomedicines · 2024Review
- Angiogenesis in the Outer Membrane of Chronic Subdural Hematomas through Thrombin-Cleaved Osteopontin and the Integrin α9 and Integrin β1 Signaling Pathways.Biomedicines · 2023Article
- TLN1 contains a cancer-associated cassette exon that alters talin-1 mechanosensitivity.The Journal of cell biology · 2023Article
- Zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface.Communications biology · 2023Article
- Tumor Suppressor DAPK1 Catalyzes Adhesion Assembly on Rigid but Anoikis on Soft Matrices.Frontiers in cell and developmental biology · 2022Article
- The actin networks of chytrid fungi reveal evolutionary loss of cytoskeletal complexity in the fungal kingdom.Current biology : CB · 2021Article
- Manipulation of Focal Adhesion Signaling by Pathogenic Microbes.International journal of molecular sciences · 2021Review
- Patient genetics is linked to chronic wound microbiome composition and healing.PLoS pathogens · 2020Article
- Feeling Things Out: Bidirectional Signaling of the Cell-ECM Interface, Implications in the Mechanobiology of Cell Spreading, Migration, Proliferation, and Differentiation.Advanced healthcare materials · 2020Review
- Filamin A mediates isotropic distribution of applied force across the actin network.The Journal of cell biology · 2019Article
- "Biological Adhesion" is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study.International journal of molecular sciences · 2019Article
- Proteomic changes of CD4Experimental and therapeutic medicine · 2017Article
- Fibronectin-bound α5β1 integrins sense load and signal to reinforce adhesion in less than a second.Nature materials · 2017Article
- Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity.The Journal of cell biology · 2016Article
- High Stoichiometry Phosphorylation of Talin at T144/T150 or S446 Produces Contrasting Effects on Calpain-mediated Talin Cleavage and Cell Migration.Journal of Cancer · 2016Article
- N-WASP-directed actin polymerization activates Cas phosphorylation and lamellipodium spreading.Journal of cell science · 2014Article
- Talins and kindlins: partners in integrin-mediated adhesion.Nature reviews. Molecular cell biology · 2013Review
- Cathepsin H mediates the processing of talin and regulates migration of prostate cancer cells.The Journal of biological chemistry · 2013Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used gene disruption to isolate two talin (-/-) ES cell mutants that contain no intact talin. The undifferentiated cells (a) were unable to spread on gelatin or laminin and grew as rounded colonies, although they were able to spread on fibronectin (b) showed reduced adhesion to laminin, but not fibronectin (c) expressed much reduced levels of beta1 integrin, although levels of alpha5 and alphaV were wild-type (d) were less polarized with increased membrane protrusions compared with a vinculin (-/-) ES cell mutant (e) were unable to assemble vinculin or paxillin-containing focal adhesions or actin stress fibers on fibronectin, whereas vinculin (-/-) ES cells were able to assemble talin-containing focal adhesions. Both talin (-/-) ES cell mutants formed embryoid bodies, but differentiation was restricted to two morphologically distinct cell types. Interestingly, these differentiated talin (-/-) ES cells were able to spread and form focal adhesion-like structures containing vinculin and paxillin on fibronectin. Moreover, the levels of the beta1 integrin subunit were comparable to those in wild-type ES cells. We conclude that talin is essential for beta1 integrin expression and focal adhesion assembly in undifferentiated ES cells, but that a subset of differentiated cells are talin independent for both characteristics.
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