ArticleThe Journal of cell biology1998
Involvement of phospholipase Cgamma1 in mouse egg activation induced by a truncated form of the C-kit tyrosine kinase present in spermatozoa.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 115 citations in OpenAlex.
- Applications of advances in mRNA-based platforms as therapeutics and diagnostics in reproductive technologies.Frontiers in cell and developmental biology · 2023Review
- Oocyte activation deficiency and assisted oocyte activation: mechanisms, obstacles and prospects for clinical application.Human reproduction open · 2022Review
- Increasing associations between defects in phospholipase C zeta and conditions of male infertility: not just ICSI failure?Journal of assisted reproduction and genetics · 2020Review
- Tr-KIT/c-KIT ratio in renal cell carcinoma.Molecular biology reports · 2019Article
- Reduction of truncated Kit Expression in Men with Abnormal Semen Parameters, Globozoospermia and History of Low or Fertilization Failure.Cell journal · 2019Article
- The stem cell factor (SCF)/c-KIT signalling in testis and prostate cancer.Journal of cell communication and signaling · 2017Article
- Article
- The transcriptional co-activator SND1 is a novel regulator of alternative splicing in prostate cancer cells.Oncogene · 2014Article
- The centrosomal kinase NEK2 is a novel splicing factor kinase involved in cell survival.Nucleic acids research · 2014Article
- Protein-tyrosine kinase signaling in the biological functions associated with sperm.Journal of signal transduction · 2012Article
- Article
- Murine hematopoietic stem cells and multipotent progenitors express truncated intracellular form of c-kit receptor.Stem cells and development · 2008Article
- The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x.The Journal of cell biology · 2007Article
- The nuclear RNA-binding protein Sam68 translocates to the cytoplasm and associates with the polysomes in mouse spermatocytes.Molecular biology of the cell · 2006Article
- Functional interaction between p90Rsk2 and Emi1 contributes to the metaphase arrest of mouse oocytes.The EMBO journal · 2004Article
- Expression of a truncated form of the c-Kit tyrosine kinase receptor and activation of Src kinase in human prostatic cancer.The American journal of pathology · 2004Article
- Tr-kit promotes the formation of a multimolecular complex composed by Fyn, PLCgamma1 and Sam68.Oncogene · 2003Article
- Tr-kit-induced resumption of the cell cycle in mouse eggs requires activation of a Src-like kinase.The EMBO journal · 2002Article
- Kit signaling and negative regulation of daunorubicin-induced apoptosis: role of phospholipase Cgamma.Oncogene · 2001Article
- Role of c-kit in mammalian spermatogenesis.Journal of endocrinological investigation · 2000Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microinjection of a truncated form of the c-kit tyrosine kinase present in mouse spermatozoa (tr-kit) activates mouse eggs parthenogenetically, and tr-kit- induced egg activation is inhibited by preincubation with an inhibitor of phospholipase C (PLC) (Sette, C., A. Bevilacqua, A. Bianchini, F. Mangia, R. Geremia, and P. Rossi. 1997. Development [Camb.]. 124:2267-2274). Co-injection of glutathione-S-transferase (GST) fusion proteins containing the src-homology (SH) domains of the gamma1 isoform of PLC (PLCgamma1) competitively inhibits tr-kit- induced egg activation. A GST fusion protein containing the SH3 domain of PLCgamma1 inhibits egg activation as efficiently as the whole SH region, while a GST fusion protein containing the two SH2 domains is much less effective. A GST fusion protein containing the SH3 domain of the Grb2 adaptor protein does not inhibit tr-kit-induced egg activation, showing that the effect of the SH3 domain of PLCgamma1 is specific. Tr-kit-induced egg activation is also suppressed by co-injection of antibodies raised against the PLCgamma1 SH domains, but not against the PLCgamma1 COOH-terminal region. In transfected COS cells, coexpression of PLCgamma1 and tr-kit increases diacylglycerol and inositol phosphate production, and the phosphotyrosine content of PLCgamma1 with respect to cells expressing PLCgamma1 alone. These data indicate that tr-kit activates PLCgamma1, and that the SH3 domain of PLCgamma1 is essential for tr-kit-induced egg activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.