ArticleThe Journal of cell biology1998
Characterization of the p22 subunit of dynactin reveals the localization of cytoplasmic dynein and dynactin to the midbody of dividing cells.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 79 citations in OpenAlex.
- A proteome-wide association study identifies putative causal proteins for breast cancer risk.British journal of cancer · 2024Article
- The splicing-factor Prp40 affects dynein-dynactin function inMolecular biology of the cell · 2020Article
- Cargo-Mediated Activation of Cytoplasmic DyneinFrontiers in cell and developmental biology · 2020Review
- Prognostic Value of Dynactin mRNA Expression in Cutaneous Melanoma.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Recurring DNA copy number gain at chromosome 9p13 plays a role in the activation of multiple candidate oncogenes in progressing oral premalignant lesions.Cancer medicine · 2014Article
- Interaction of foot-and-mouth disease virus nonstructural protein 3A with host protein DCTN3 is important for viral virulence in cattle.Journal of virology · 2014Article
- The dynactin complex maintains the integrity of metaphasic centrosomes to ensure transition to anaphase.The Journal of biological chemistry · 2011Article
- Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin.Genes to cells : devoted to molecular & cellular mechanisms · 2010Article
- Imipramine treatment and resiliency exhibit similar chromatin regulation in the mouse nucleus accumbens in depression models.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- RACK-1 directs dynactin-dependent RAB-11 endosomal recycling during mitosis in Caenorhabditis elegans.Molecular biology of the cell · 2009Article
- Kinesin and dynein-dynactin at intersecting microtubules: motor density affects dynein function.Biophysical journal · 2008Article
- The Saccharomyces cerevisiae homolog of p24 is essential for maintaining the association of p150Glued with the dynactin complex.Genetics · 2008Article
- Breast cancer molecular signatures as determined by SAGE: correlation with lymph node status.Molecular cancer research : MCR · 2007Article
- Cytoplasmic dynein's mitotic spindle pole localization requires a functional anaphase-promoting complex, gamma-tubulin, and NUDF/LIS1 in Aspergillus nidulans.Molecular biology of the cell · 2005Article
- Inhibition of prostate tumor growth by overexpression of NudC, a microtubule motor-associated protein.Oncogene · 2004Article
- An automated in vitro protein folding screen applied to a human dynactin subunit.Protein science : a publication of the Protein Society · 2004Article
- Analysis of the dynein-dynactin interaction in vitro and in vivo.Molecular biology of the cell · 2003Article
- The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization.Molecular biology of the cell · 2003Article
- Distinct cell cycle-dependent roles for dynactin and dynein at centrosomes.The Journal of cell biology · 2002Article
- PLAC-24 is a cytoplasmic dynein-binding protein that is recruited to sites of cell-cell contact.Molecular biology of the cell · 2002Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Dynactin, a multisubunit complex that binds to the microtubule motor cytoplasmic dynein, may provide a link between dynein and its cargo. Many subunits of dynactin have been characterized, elucidating the multifunctional nature of this complex. Using a dynein affinity column, p22, the smallest dynactin subunit, was isolated and microsequenced. The peptide sequences were used to clone a full-length human cDNA. Database searches with the predicted amino acid sequence of p22 indicate that this polypeptide is novel. We have characterized p22 as an integral component of dynactin by biochemical and immunocytochemical methods. Affinity chromatography experiments indicate that p22 binds directly to the p150(Glued) subunit of dynactin. Immunocytochemistry with antibodies to p22 demonstrates that this polypeptide localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Antibodies to p22, as well as to other dynactin subunits, also revealed a novel localization for dynactin to the cleavage furrow and to the midbodies of dividing cells; cytoplasmic dynein was also localized to these structures. We therefore propose that dynein/dynactin complexes may have a novel function during cytokinesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.