ArticleThe Journal of cell biology1998
Mechanism of Cdc42-induced actin polymerization in neutrophil extracts.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 69 citations in OpenAlex.
- Lateral distribution of phosphatidylinositol 4,5-bisphosphate in membranes regulates formin- and ARP2/3-mediated actin nucleation.The Journal of biological chemistry · 2019Article
- Internetwork competition for monomers governs actin cytoskeleton organization.Nature reviews. Molecular cell biology · 2016Article
- Critical role of actin-associated proteins in smooth muscle contraction, cell proliferation, airway hyperresponsiveness and airway remodeling.Respiratory research · 2015Review
- Homeostatic actin cytoskeleton networks are regulated by assembly factor competition for monomers.Current biology : CB · 2014Article
- Regulation of conformer-specific activation of the integrin LFA-1 by a chemokine-triggered Rho signaling module.Nature immunology · 2009Article
- Cell biology of embryonic migration.Birth defects research. Part C, Embryo today : reviews · 2008Review
- Physiologic properties and regulation of the actin cytoskeleton in vascular smooth muscle.Journal of cardiovascular pharmacology and therapeutics · 2008Review
- Article
- Growth of branched actin networks against obstacles.Biophysical journal · 2001Article
- Isolation and characterization of effector-loop mutants of CDC42 in yeast.Molecular biology of the cell · 2001Article
- A novel neural Wiskott-Aldrich syndrome protein (N-WASP) binding protein, WISH, induces Arp2/3 complex activation independent of Cdc42.The Journal of cell biology · 2001Article
- Profilin enhances Cdc42-induced nucleation of actin polymerization.The Journal of cell biology · 2000Article
- Stimulation of fascin spikes by thrombospondin-1 is mediated by the GTPases Rac and Cdc42.The Journal of cell biology · 2000Article
- Two pathways through Cdc42 couple the N-formyl receptor to actin nucleation in permeabilized human neutrophils.The Journal of cell biology · 2000Article
- Postsynaptic scaffolds of excitatory and inhibitory synapses in hippocampal neurons: maintenance of core components independent of actin filaments and microtubules.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Polarization of chemoattractant receptor signaling during neutrophil chemotaxis.Science (New York, N.Y.) · 2000Article
- Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerization.The Journal of cell biology · 2000Article
- A role for myosin-I in actin assembly through interactions with Vrp1p, Bee1p, and the Arp2/3 complex.The Journal of cell biology · 2000Article
- ARF6 is required for growth factor- and rac-mediated membrane ruffling in macrophages at a stage distal to rac membrane targeting.Molecular and cellular biology · 1999Article
- The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Cdc42, activated with GTPgammaS, induces actin polymerization in supernatants of lysed neutrophils. This polymerization, like that induced by agonists, requires elongation at filament barbed ends. To determine if creation of free barbed ends was sufficient to induce actin polymerization, free barbed ends in the form of spectrin-actin seeds or sheared F-actin filaments were added to cell supernatants. Neither induced polymerization. Furthermore, the presence of spectrin-actin seeds did not increase the rate of Cdc42-induced polymerization, suggesting that the presence of Cdc42 did not facilitate polymerization from spectrin-actin seeds such as might have been the case if Cdc42 inhibited capping or released G-actin from a sequestered pool. Electron microscopy revealed that Cdc42-induced filaments elongated rapidly, achieving a mean length greater than 1 micron in 15 s. The mean length of filaments formed from spectrin-actin seeds was <0.4 micron. Had spectrin-actin seeds elongated at comparable rates before they were capped, they would have induced longer filaments. There was little change in mean length of Cdc42-induced filaments between 15 s and 5 min, suggesting that the increase in F-actin over this time was due to an increase in filament number. These data suggest that Cdc42 induction of actin polymerization requires both creation of free barbed ends and facilitated elongation at these ends.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.