ArticleThe Journal of cell biology1998
Microinjection of anti-coilin antibodies affects the structure of coiled bodies.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
58 citing papers in PubMed, 87 citations in OpenAlex.
- Dynamic interaction of spliceosomal snRNPs with coilin explains Cajal body characteristics.The Journal of cell biology · 2025Article
- The coilin N-terminus mediates multivalent interactions between coilin and Nopp140 to form and maintain Cajal bodies.Nature communications · 2022Article
- Keeping up with the condensates: The retention, gain, and loss of nuclear membrane-less organelles.Frontiers in molecular biosciences · 2022Review
- Nopp140-mediated concentration of telomerase in Cajal bodies regulates telomere length.Molecular biology of the cell · 2019Article
- Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.The Journal of cell biology · 2017Article
- Enhanced snoMEN Vectors Facilitate Establishment of GFP-HIF-1α Protein Replacement Human Cell Lines.PloS one · 2016Article
- Analysis of human protein replacement stable cell lines established using snoMEN-PR vector.PloS one · 2013Article
- Article
- A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly.Nature cell biology · 2011Article
- In vivo kinetics of U4/U6·U5 tri-snRNP formation in Cajal bodies.Molecular biology of the cell · 2011Article
- The nuclear ATPase/adenylate kinase hCINAP is recruited to perinucleolar caps generated upon RNA pol.II inhibition.FEBS letters · 2010Article
- TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo.Molecular and cellular biology · 2010Article
- Phosphorylation and the Cajal body: modification in search of function.Archives of biochemistry and biophysics · 2010Review
- Hsp70 chaperones and type I PRMTs are sequestered at intranuclear inclusions caused by polyalanine expansions in PABPN1.PloS one · 2009Article
- Nuclear pore complex proteins mark the implantation window in human endometrium.Journal of cell science · 2008Article
- Spliceosomal small nuclear ribonucleoprotein particles repeatedly cycle through Cajal bodies.Molecular biology of the cell · 2008Article
- Splicing factors SF1 and U2AF associate in extraspliceosomal complexes.Molecular and cellular biology · 2008Article
- Dephosphorylation of survival motor neurons (SMN) by PPM1G/PP2Cgamma governs Cajal body localization and stability of the SMN complex.The Journal of cell biology · 2007Article
- Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit.Molecular biology of the cell · 2007Article
- Dynamic association and localization of human H/ACA RNP proteins.RNA (New York, N.Y.) · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The coiled body is a distinct subnuclear domain enriched in small nuclear ribonucleoprotein particles (snRNPs) involved in processing of pre-mRNA. Although the function of the coiled body is still unknown, current models propose that it may have a role in snRNP biogenesis, transport, or recycling. Here we describe that anti-coilin antibodies promote a specific disappearance of the coiled body in living human cells, thus providing a novel tool for the functional analysis of this structure. Monoclonal antibodies (mAbs) were raised against recombinant human coilin, the major structural protein of the coiled body. Four mAbs are shown to induce a progressive disappearance of coiled bodies within approximately 6 h after microinjection into the nucleus of HeLa cells. After their disappearance, coiled bodies are not seen to re-form, although injected cells remain viable for at least 3 d. Epitope mapping reveals that the mAbs recognize distinct amino acid motifs scattered along the complete coilin sequence. By 24 and 48 h after injection of antibodies that promote coiled body disappearance, splicing snRNPs are normally distributed in the nucleoplasm, the nucleolus remains unaffected, and the cell cycle progresses normally. Furthermore, cells devoid of coiled bodies for approximately 24 h maintain the ability to splice both adenoviral pre-mRNAs and transiently overexpressed human beta-globin transcripts. In conclusion, within the time range of this study, no major nuclear abnormalities are detected after coiled body disappearance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.