ArticleJournal of bacteriology1998
Pas, a novel protein required for protein secretion and attaching and effacing activities of enterohemorrhagic Escherichia coli.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Physiological and Transcriptomic Analyses ofMicroorganisms · 2023Article
- Transposon-insertion sequencing screens unveil requirements for EHEC growth and intestinal colonization.PLoS pathogens · 2019Article
- Type Three Secretion System in Attaching and Effacing Pathogens.Frontiers in cellular and infection microbiology · 2016Review
- The inhibitory effect of colloidal bismuth hydroxide gel on Escherichia coli O157:H7 and on the activity of Shiga toxins.BMC research notes · 2014Article
- Structure and interactions of the cytoplasmic domain of the Yersinia type III secretion protein YscD.Journal of bacteriology · 2012Article
- Escherichia coli O157:H7-Clinical aspects and novel treatment approaches.Frontiers in cellular and infection microbiology · 2012Review
- Transcriptomic response of Escherichia coli O157:H7 to oxidative stress.Applied and environmental microbiology · 2009Article
- Interactions between CdsD, CdsQ, and CdsL, three putative Chlamydophila pneumoniae type III secretion proteins.Journal of bacteriology · 2008Article
- Active genetic elements present in the locus of enterocyte effacement in Escherichia coli O26 and their role in mobility.Infection and immunity · 2006Article
- Bioinformatics analysis of the locus for enterocyte effacement provides novel insights into type-III secretion.BMC microbiology · 2005Article
- The locus of enterocyte effacement-encoded effector proteins all promote enterohemorrhagic Escherichia coli pathogenicity in infant rabbits.Infection and immunity · 2005Article
- Dissecting virulence: systematic and functional analyses of a pathogenicity island.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Translocated intimin receptor and its chaperone interact with ATPase of the type III secretion apparatus of enteropathogenic Escherichia coli.Journal of bacteriology · 2003Article
- Characterization of SepL of enterohemorrhagic Escherichia coli.Journal of bacteriology · 2000Article
- Isolation and characterization of mini-Tn5Km2 insertion mutants of enterohemorrhagic Escherichia coli O157:H7 deficient in adherence to Caco-2 cells.Infection and immunity · 2000Article
- Molecular characteristics and epidemiological significance of Shiga toxin-producing Escherichia coli O26 strains.Journal of clinical microbiology · 2000Article
- The EspD protein of enterohemorrhagic Escherichia coli is required for the formation of bacterial surface appendages and is incorporated in the cytoplasmic membranes of target cells.Infection and immunity · 1999Article
- Transcriptional regulation of the esp genes of enterohemorrhagic Escherichia coli.Journal of bacteriology · 1999Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterohemorrhagic Escherichia coli (EHEC) exhibits a pattern of localized adherence to host cells, with the formation of microcolonies, and induces a specific histopathological phenotype collectively known as the attaching and effacing lesion. The genes encoding the products responsible for this phenotype are located on a 35-kb pathogenicity island designated the locus of enterocyte effacement, which is also shared by enteropathogenic E. coli. We have identified an open reading frame (ORF) which is located upstream of the espA, espB, and espD genes on the complementary strand and which exhibits high homology to the genes spiB from Salmonella, yscD from Yersinia, and pscD from Pseudomonas. Localization studies showed that the encoded product is present in the cytoplasmic and inner membrane fractions of EHEC. The construction and characterization of a recombinant clone containing an in-frame deletion of this ORF demonstrated that the encoded product is a putative member of a type III system required for protein secretion. Disruption of this ORF, designated pas (protein associated with secretion), abolished the secretion of Esp proteins. The mutant adhered only poorly and lost its capacities to trigger attaching and effacing activity and to invade HeLa cells. These results demonstrate that Pas is a virulence-associated factor that plays an essential role in EHEC pathogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.