ArticleThe Journal of physiology1998
A characterization of muscarinic receptor-mediated intracellular Ca2+ mobilization in cultured rat hippocampal neurones.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 66 citations in OpenAlex.
- Neuronal Store-Operated Calcium Channels.Molecular neurobiology · 2023Review
- Involvement of CaCellular and molecular neurobiology · 2023Article
- Store-operated calcium entry promotes the degradation of the transcription factor Sp4 in resting neurons.Science signaling · 2014Article
- Carbachol-induced long-term synaptic depression is enhanced during senescence at hippocampal CA3-CA1 synapses.Journal of neurophysiology · 2010Article
- Inositol trisphosphate 3-kinases: focus on immune and neuronal signaling.Cellular and molecular life sciences : CMLS · 2010Review
- Silencing the SPCA1 (secretory pathway Ca2+-ATPase isoform 1) impairs Ca2+ homeostasis in the Golgi and disturbs neural polarity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Role of STIM1 in regulation of store-operated Ca2+ influx in pheochromocytoma cells.Cellular and molecular neurobiology · 2009Article
- Cannabidiol targets mitochondria to regulate intracellular Ca2+ levels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Calcium-independent phospholipase A2 mediates store-operated calcium entry in rat cerebellar granule cells.Cerebellum (London, England) · 2008Article
- Expansion of the calcium hypothesis of brain aging and Alzheimer's disease: minding the store.Aging cell · 2007Review
- Receptor-specific inhibition of GABAB-activated K+ currents by muscarinic and metabotropic glutamate receptors in immature rat hippocampus.The Journal of physiology · 2007Article
- Modulation of Gq-protein-coupled inositol trisphosphate and Ca2+ signaling by the membrane potential.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Molecular mechanisms of Ca(2+) signaling in neurons induced by the S100A4 protein.Molecular and cellular biology · 2006Article
- Neural cell adhesion molecule induces intracellular signaling via multiple mechanisms of Ca2+ homeostasis.Molecular biology of the cell · 2006Article
- The Kv2.1 C terminus can autonomously transfer Kv2.1-like phosphorylation-dependent localization, voltage-dependent gating, and muscarinic modulation to diverse Kv channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Synaptically activated ca2+ release from internal stores in CNS neurons.Cellular and molecular neurobiology · 2005Review
- Intracellular calcium store filling by an L-type calcium current in the basolateral amygdala at subthreshold membrane potentials.The Journal of physiology · 2005Article
- Ginseng saponins induce store-operated calcium entry in Xenopus oocytes.British journal of pharmacology · 2004Article
- Activity-evoked capacitative Ca2+ entry: implications in synaptic plasticity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Leptin inhibits epileptiform-like activity in rat hippocampal neurones via PI 3-kinase-driven activation of BK channels.The Journal of physiology · 2002Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
1. The properties of muscarinic receptor-mediated Ca2+ mobilization were investigated in hippocampal cultures using fluorescent imaging techniques. 2. Somatic responses to carbachol (1-10 microM) were observed in 21 % of neurones under control conditions (5.4 mM K+, 1. 8 mM Ca2+, 0.5-1 microM tetrodotoxin). Smaller responses were observed in Ca2+-free medium. 3. In cells where responses to carbachol were absent under control conditions, responses were often observed following depolarization with high extracellular K+ (16. 2-25 mM). These responses decreased in magnitude with time after the depolarizing episode. Mobilization of Ca2+ from stores using caffeine (50 mM) exhibited similar properties. 4. Carbachol responses were greatly facilitated in the presence of moderate elevations in extracellular K+ or Ca2+ levels (2- or 3-fold, respectively). These conditions were usually, but not always, associated with a small increase in cytosolic Ca2+ levels (< 50 nM). 5. Muscarinic responses in 10.8 mM K+ were inhibited by 80-95 % in the presence of the L-type voltage-gated Ca2+ channel antagonists nitrendipine (2-5 microM) or nifedipine (10 microM). Depletion of intracellular Ca2+ stores with thapsigargin (2-10 microM) blocked responses. 6. Oscillatory Ca2+ mobilizing responses were observed in some cells. Their expression was facilitated by moderate cytosolic Ca2+ elevations and by increasing the duration of carbachol exposure. 7. Ca2+ mobilizing responses were also observed in dendritic regions. These were smaller than somatic responses, but had faster decay kinetics. 8. In conclusion, muscarinic receptor-mediated Ca2+ mobilization in cultured hippocampal neurones shows a strong Ca2+ dependence. Moderate intracellular Ca2+ rises greatly facilitate muscarinic responses and uncover, in some cells, oscillatory Ca2+ mobilization. These effects appear to reflect the loading state of intracellular Ca2+ stores.
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