ArticleThe Journal of physiology1998
Intracellular nucleotide-mediated gating of SUR/Kir6.0 complex potassium channels expressed in a mammalian cell line and its modification by pinacidil.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 65 citations in OpenAlex.
- GLP-1 activates KNature communications · 2026Article
- Electro-metabolic signaling.The Journal of general physiology · 2024Article
- Mechanistic insights on KATP channel regulation from cryo-EM structures.The Journal of general physiology · 2023Review
- Involvement of SUR2/Kir6.1 channel in the physiopathology of pulmonary arterial hypertension.Frontiers in cardiovascular medicine · 2022Article
- Vascular KProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Adenosine Triphosphate-Sensitive Potassium Currents in Heart Disease and Cardioprotection.Cardiac electrophysiology clinics · 2016Review
- The shifting landscape of KATP channelopathies and the need for 'sharper' therapeutics.Future medicinal chemistry · 2016Review
- KATP channels and cardiovascular disease: suddenly a syndrome.Circulation research · 2013Review
- Interaction with caveolin-1 modulates vascular ATP-sensitive potassium (KATP) channel activity.The Journal of physiology · 2010Article
- Muscle KATP channels: recent insights to energy sensing and myoprotection.Physiological reviews · 2010Review
- Substitution of the Walker A lysine by arginine in the nucleotide-binding domains of sulphonylurea receptor SUR2B: effects on ligand binding and channel activity.Naunyn-Schmiedeberg's archives of pharmacology · 2010Article
- Review
- Analysis of two KCNJ11 neonatal diabetes mutations, V59G and V59A, and the analogous KCNJ8 I60G substitution: differences between the channel subtypes formed with SUR1.The Journal of biological chemistry · 2009Article
- PKA phosphorylation of SUR2B subunit underscores vascular KATP channel activation by beta-adrenergic receptors.American journal of physiology. Regulatory, integrative and comparative physiology · 2007Article
- Selectivity of repaglinide and glibenclamide for the pancreatic over the cardiovascular K(ATP) channels.Diabetologia · 2006Article
- ATP-sensitive K+ channel channel/enzyme multimer: metabolic gating in the heart.Journal of molecular and cellular cardiology · 2005Review
- Differential expression of Kir6.1 and SUR2B mRNAs in the vasculature of various tissues in rats.The Journal of membrane biology · 2003Article
- Binding and effect of K ATP channel openers in the absence of Mg2+.British journal of pharmacology · 2003Article
- Protein kinase C modulation of recombinant ATP-sensitive K(+) channels composed of Kir6.1 and/or Kir6.2 expressed with SUR2B.The Journal of physiology · 2002Article
- Effects of KRN2391 on ionic currents in rabbit femoral arterial myocytes.British journal of pharmacology · 2001Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. We have examined the properties of intracellular nucleotide-mediated gating of K+ channel constructs composed of the sulphonylurea receptor 2B and the inwardly rectifying K+ channel subunits Kir6.1 and Kir6.2 (SUR2B/Kir6.1 and SUR2B/Kir6.2 complex K+ channels) heterologously expressed in human embryonic kidney (HEK) 293T cells. In the cell-attached form, both types of K+ channel were activated by pinacidil. 2. In inside-out (IO) patches, the SUR2B/Kir6.2 channels opened spontaneously and were inhibited by intracellular ATP (ATPi). Pinacidil attenuated the ATPi-mediated channel inhibition in a concentration-dependent manner. In contrast, the SUR2B/Kir6.1 channels required intracellular nucleoside di- or tri-, but not mono-, phosphates for opening. The potency of adenine, guanine or uracil nucleotides to activate SUR2B/Kir6.1 channels was enhanced by pinacidil. 3. In the presence of pinacidil, adenine and guanine, but not uracil, nucleotides exhibited bell-shaped concentration-dependent activating effects on SUR2B/Kir6.1 channels. This was due to channel inhibition caused by adenine and guanine nucleotides, which was unaffected by pinacidil. 4. From power density spectrum analysis of SUR2B/Kir6.1 currents, channel activation could be described by the product of two gates, a nucleotide-independent fast channel gate and a nucleotide-dependent slow gate, which controlled the number of functional channels. Pinacidil specifically increased the potency of nucleotide action on the slow gate. 5. We conclude that Kir6.0 subunits play a crucial role in the nucleotide-mediated gating of SUR/Kir6.0 complex K+ channels and may determine the molecular mode of pinacidil action.
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