ArticleInfection and immunity1998
Early events in phagosome establishment are required for intracellular survival of Legionella pneumophila.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 124 citations in OpenAlex.
- Article
- Non-canonical activation of the ER stress sensor ATF6 byLife science alliance · 2021Article
- Components of the endocytic and recycling trafficking pathways interfere with the integrity of the Legionella-containing vacuole.Cellular microbiology · 2020Article
- Interfering with Autophagy: The Opposing Strategies Deployed byFrontiers in cellular and infection microbiology · 2020Review
- Review
- KKL-35 Exhibits Potent Antibiotic Activity against Legionella Species Independently ofAntimicrobial agents and chemotherapy · 2018Article
- A Single Legionella Effector Catalyzes a Multistep Ubiquitination Pathway to Rearrange Tubular Endoplasmic Reticulum for Replication.Cell host & microbe · 2017Article
- Beyond Paralogs: The Multiple Layers of Redundancy in Bacterial Pathogenesis.Frontiers in cellular and infection microbiology · 2017Review
- PilY1 PromotesFrontiers in cellular and infection microbiology · 2017Article
- Article
- Endoplasmic Reticulum Tubule Protein Reticulon 4 Associates with the Legionella pneumophila Vacuole and with Translocated Substrate Ceg9.Infection and immunity · 2015Article
- Legionella pneumophila LidA affects nucleotide binding and activity of the host GTPase Rab1.Journal of bacteriology · 2012Article
- The E Block motif is associated with Legionella pneumophila translocated substrates.Cellular microbiology · 2011Article
- Cyclic diguanylate signaling proteins control intracellular growth of Legionella pneumophila.mBio · 2011Article
- Dot/Icm type IVB secretion system requirements for Coxiella burnetii growth in human macrophages.mBio · 2011Article
- Lipidation by the host prenyltransferase machinery facilitates membrane localization of Legionella pneumophila effector proteins.The Journal of biological chemistry · 2010Article
- Chemical genetics reveals bacterial and host cell functions critical for type IV effector translocation by Legionella pneumophila.PLoS pathogens · 2009Article
- Caspase-7 activation by the Nlrc4/Ipaf inflammasome restricts Legionella pneumophila infection.PLoS pathogens · 2009Article
- Modulation of ubiquitin dynamics and suppression of DALIS formation by the Legionella pneumophila Dot/Icm system.Cellular microbiology · 2009Article
- Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence.Cellular microbiology · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
During infection, the Legionnaires' disease bacterium, Legionella pneumophila, survives and multiplies within a specialized phagosome that is near neutral pH and does not fuse with host lysosomes. In order to understand the molecular basis of this organism's ability to control its intracellular fate, we have isolated and characterized a group of transposon-generated mutants which were unable to kill macrophages and were subsequently found to be defective in intracellular multiplication. These mutations define a set of 20 genes (19 icm [for intracellular multiplication] genes and dotA [for defect in organelle trafficking]). In this report, we describe a quantitative assay for phagosome-lysosome fusion (PLF) and its use to measure the levels of PLF in cells that have been infected with either wild-type L. pneumophila or one of several mutants defective in different icm genes or dotA. By using quantitative confocal fluorescence microscopy, PLF could be scored on a per-bacterium basis by determining the extent to which fluorescein-labeled L. pneumophila colocalized with host lysosomes prelabeled with rhodamine-dextran. Remarkably, mutations in the six genes that were studied resulted in maximal levels of PLF as quickly as 30 min following infection. These results indicate that several, and possibly all, of the icm and dotA gene products act at an early step during phagosome establishment to determine whether L. pneumophila-containing phagosomes will fuse with lysosomes. Although not ruled out, subsequent activity of these gene products may not be necessary for successful intracellular replication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.