ArticleInfection and immunity1998
Deletion of the central proline-rich repeat domain results in altered antigenicity and lack of surface expression of the Streptococcus mutans P1 adhesin molecule.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
- Structure-Function Characterization of Streptococcus intermedius Surface Antigen Pas.Journal of bacteriology · 2021Article
- The Combinations Chitosan-PamInfection and immunity · 2019Article
- Coccidioidomycosis: Epidemiology, Fungal Pathogenesis, and Therapeutic Development.Current tropical medicine reports · 2019Article
- Flagellin-rPAc vaccine inhibits biofilm formation but not proliferation of S. mutans.Human vaccines & immunotherapeutics · 2016Article
- Identification of a supramolecular functional architecture of Streptococcus mutans adhesin P1 on the bacterial cell surface.The Journal of biological chemistry · 2015Article
- An intramolecular lock facilitates folding and stabilizes the tertiary structure of Streptococcus mutans adhesin P1.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Intranasal co-delivery of IL-6 gene enhances the immunogenicity of anti-caries DNA vaccine.Acta pharmacologica Sinica · 2014Article
- Alterations in immunodominance of Streptococcus mutans AgI/II: lessons learned from immunomodulatory antibodies.Vaccine · 2014Article
- An intramolecular interaction involving the N terminus of a streptococcal adhesin affects its conformation and adhesive function.The Journal of biological chemistry · 2013Article
- Functional amyloid formation by Streptococcus mutans.Microbiology (Reading, England) · 2012Article
- Article
- Crystal structure of the C-terminal region of Streptococcus mutans antigen I/II and characterization of salivary agglutinin adherence domains.The Journal of biological chemistry · 2011Article
- Mechanism of adhesion maintenance by methionine sulphoxide reductase in Streptococcus gordonii.Molecular microbiology · 2011Article
- A mechanism for extremely weak SpaP-expression in Streptococcus mutans strain Z1.Journal of oral microbiology · 2011Article
- Induction of neutralizing antibodies in mice immunized with an amino-terminal polypeptide of Streptococcus mutans P1 protein produced by a recombinant Bacillus subtilis strain.FEMS immunology and medical microbiology · 2010Article
- The changing faces of Streptococcus antigen I/II polypeptide family adhesins.Molecular microbiology · 2010Review
- Beneficial immunomodulation by Streptococcus mutans anti-P1 monoclonal antibodies is Fc independent and correlates with increased exposure of a relevant target epitope.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Requirements for surface expression and function of adhesin P1 from Streptococcus mutans.Infection and immunity · 2008Article
- Evaluation of two homologous proline-rich proteins of Coccidioides posadasii as candidate vaccines against coccidioidomycosis.Infection and immunity · 2007Article
- Identification and characterization of an antigen I/II family protein produced by group A Streptococcus.Infection and immunity · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Members of the family of surface adhesins of oral streptococci, including P1 of Streptococcus mutans, contain two highly conserved repeat domains, one rich in alanine (A region) and the other rich in proline (P region). To assess the contribution of the P region to the biological properties of P1, an internal deletion in spaP was engineered. In addition, the P region was subcloned and expressed as a fusion partner with the maltose binding protein of Escherichia coli and liberated by digestion with factor Xa. Results of Western blot experiments in which recombinant polypeptides were probed with a panel of 11 monoclonal antibodies indicated that the P region is a necessary component of conformational epitopes within the central portion of P1. Antibodies reactive with the P region were detected in a polyclonal rabbit antiserum generated against whole S. mutans cells but not in two rabbit antisera generated against purified P1 (Mr approximately 185,000), suggesting that this domain is immunogenic on the surface of intact bacteria but not as part of a soluble full-length molecule. Finally, transformation of a spaP-negative mutant with a shuttle vector containing an internally deleted spaP lacking P-region DNA resulted in a complete absence of surface-localized P1 and substantially less P1 in sonicated cells compared to the case for the mutant complemented with the full-length gene. These results suggest that the P region is an integral component contributing to the conformation of the central region of P1 and indicate that its presence is necessary for surface expression of the molecule on S. mutans.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.