ArticleInfection and immunity1998
Characterization of major surface glycoprotein genes of human Pneumocystis carinii and high-level expression of a conserved region.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Axenic Long-Term Cultivation ofJournal of fungi (Basel, Switzerland) · 2023Article
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- Serological diagnosis of pneumocystosis: production of a synthetic recombinant antigen for immunodetection of Pneumocystis jirovecii.Scientific reports · 2016Article
- Distinguishing highly similar gene isoforms with a clustering-based bioinformatics analysis of PacBio single-molecule long reads.BioData mining · 2016Article
- Antibody responses against Pneumocystis jirovecii in health care workers over time.Emerging infectious diseases · 2013Article
- Expression of Pneumocystis jirovecii major surface glycoprotein in Saccharomyces cerevisiae.The Journal of infectious diseases · 2013Article
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- Discordant antibody and cellular responses to Pneumocystis major surface glycoprotein variants in mice.BMC immunology · 2012Article
- Colonization by Pneumocystis jirovecii and its role in disease.Clinical microbiology reviews · 2012Review
- Serologic responses to recombinant Pneumocystis jirovecii major surface glycoprotein among Ugandan patients with respiratory symptoms.PloS one · 2012Article
- Serologic responses to pneumocystis proteins in HIV patients with and without Pneumocystis jirovecii pneumonia.Journal of acquired immune deficiency syndromes (1999) · 2011Article
- Healthcare worker occupation and immune response to Pneumocystis jirovecii.Emerging infectious diseases · 2009Article
- Geographical variation in serological responses to recombinant Pneumocystis jirovecii major surface glycoprotein antigens.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2009Article
- Long-term serologic responses to the Pneumocystis jirovecii major surface glycoprotein in HIV-positive individuals with and without P. jirovecii infection.The Journal of infectious diseases · 2009Article
- Variation in the major surface glycoprotein genes in Pneumocystis jirovecii.The Journal of infectious diseases · 2008Article
- Human immunodeficiency virus-infected patients with prior Pneumocystis pneumonia exhibit increased serologic reactivity to several major surface glycoprotein clones.Clinical and vaccine immunology : CVI · 2006Article
- Antibody response to Pneumocystis jirovecii major surface glycoprotein.Emerging infectious diseases · 2006Article
- Serodiagnosis of mycoses using recombinant antigens.Mycopathologia · 2005Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To facilitate studies of Pneumocystis carinii infection in humans, we undertook to better characterize and to express the major surface glycoprotein (MSG) of human P. carinii, an important protein in host-pathogen interactions. Seven MSG genes were cloned from a single isolate by PCR or genomic library screening and were sequenced. The predicted proteins, like rat MSGs, were closely related but unique variants, with a high level of conservation among cysteine residues. A conserved immunodominant region (of approximately 100 amino acids) near the carboxy terminus was expressed at high levels in Escherichia coli and used in Western blot studies. All 49 of the serum samples, which were taken from healthy controls as well as from patients with and without P. carinii pneumonia, were reactive with this peptide by Western blotting, supporting the hypothesis that most adult humans have been infected with P. carinii at some point. This recombinant MSG fragment, which is the first human P. carinii antigen available in large quantities, may be a useful reagent for investigating the epidemiology of P. carinii infection in humans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.