ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
Authentic cell-specific and developmentally regulated expression of pro-opiomelanocortin genomic fragments in hypothalamic and hindbrain neurons of transgenic mice.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed, 79 citations in OpenAlex.
- Significance of neuroendocrine systems and the gut-brain axis (GBA) in the regulation of obesity.PeerJ · 2026Review
- Epigenetic modification of hypothalamic neuropeptides and metabolic hormone receptors in metabolic health.Frontiers in endocrinology · 2025Review
- The homeodomain transcription factor Six3 regulates hypothalamic Pomc expression and its absence from POMC neurons induces hyperphagia and mild obesity in male mice.Molecular metabolism · 2024Article
- Appetite- and Weight-Regulating Neuroendocrine Circuitry in Hypothalamic Obesity.Endocrine reviews · 2024Review
- POMC-specific knockdown of MeCP2 leads to adverse phenotypes in mice chronically exposed to high fat diet.Behavioural brain research · 2024Article
- Adult-born proopiomelanocortin neurons derived from Rax-expressing precursors mitigate the metabolic effects of congenital hypothalamic proopiomelanocortin deficiency.Molecular metabolism · 2021Article
- Mice lacking PC1/3 expression in POMC-expressing cells do not develop obesity.Endocrinology · 2021Article
- Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases.Transgenic research · 2021Article
- Role of the Melanocortin System in the Central Regulation of Cardiovascular Functions.Frontiers in physiology · 2021Review
- Oral supplementation with ginseng polysaccharide promotes food intake in mice.Brain and behavior · 2019Article
- The Homeodomain Transcription Factor NKX2.1 Is Essential for the Early Specification of Melanocortin Neuron Identity and ActivatesThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Molecular and functional genetics of the proopiomelanocortin gene, food intake regulation and obesity.FEBS letters · 2017Review
- Calorie-induced ER stress suppresses uroguanylin satiety signaling in diet-induced obesity.Nutrition & diabetes · 2016Article
- Cross-talk between reproduction and energy homeostasis: central impact of estrogens, leptin and kisspeptin signaling.Hormone molecular biology and clinical investigation · 2014Review
- Prolyl carboxypeptidase mRNA expression in the mouse brain.Brain research · 2014Article
- Enhancer turnover and conserved regulatory function in vertebrate evolution.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Prolyl carboxypeptidase and its inhibitors in metabolism.Trends in endocrinology and metabolism: TEM · 2013Article
- Positive selection of co-opted mobile genetic elements in a mammalian gene: If you can't beat them, join them.Mobile genetic elements · 2012Article
- Reciprocal regulation of TREK-1 channels by arachidonic acid and CRH in mouse corticotropes.Endocrinology · 2011Article
- A pituitary-specific enhancer of the POMC gene with preferential activity in corticotrope cells.Molecular endocrinology (Baltimore, Md.) · 2011Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pro-opiomelanocortin (POMC) gene is expressed in a subset of hypothalamic and hindbrain neurons and in pituitary melanotrophs and corticotrophs. POMC neurons release the potent opioid beta-endorphin and several active melanocortins that control homeostasis and feeding behavior. POMC gene expression in the CNS is believed to be controlled by distinct cis-acting regulatory sequences. To analyze the transcriptional regulation of POMC in neuronal and endocrine cells, we produced transgenic mice carrying POMC27*, a transgene containing the entire 6 kb of the POMC transcriptional unit together with 13 kb of 5' flanking regions and 8 kb of 3' flanking regions. POMC27* was tagged with a heterologous 30 bp oligonucleotide in the third exon. In situ hybridization studies showed an accurate cell-specific pattern of expression of POMC27* in the arcuate nucleus and the pituitary. Hypothalamic mRNA-positive neurons colocalized entirely with beta-endorphin immunoreactivity. No ectopic transgenic expression was detected in the brain. Deletional analyses demonstrated that neuron-specific expression of POMC transgenes required distal 5' sequences localized upstream of the pituitary-responsive proximal cis-acting elements that were identified previously. POMC27* exhibited a spatial and temporal pattern of expression throughout development that exactly paralleled endogenous POMC. RNase protection assays revealed that POMC27* expression mimicked that of POMC in different areas of the CNS and most peripheral organs with no detectable ectopic expression. Hormonal regulation of POMC27* and POMC was identical in the hypothalamus and pituitary. These results show that distal 5' sequences of the POMC gene located between -13 and -2 kb target expression into the CNS of transgenic mice in a precise neuron-specific, developmentally and hormonally regulated manner.
Indexed as
Identifiers
9712635PMC6792967W2167008588What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.