ArticleMolecular and cellular biology1998
Sir proteins, Rif proteins, and Cdc13p bind Saccharomyces telomeres in vivo.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 129 citations in OpenAlex.
- Review
- Unwrap RAP1's Mystery at Kinetoplastid Telomeres.Biomolecules · 2024Review
- Suppression of telomere capping defects of Saccharomyces cerevisiae yku70 and yku80 mutants by telomerase.G3 (Bethesda, Md.) · 2021Article
- Telomerase subunit Est2 marks internal sites that are prone to accumulate DNA damage.BMC biology · 2021Article
- Novel insights into the mechanism of cell cycle kinases Mec1(ATR) and Tel1(ATM).Critical reviews in biochemistry and molecular biology · 2021Article
- Article
- Back to the future: The intimate and evolving connection between telomere-related factors and genotoxic stress.The Journal of biological chemistry · 2019Review
- Rif1 phosphorylation site analysis in telomere length regulation and the response to damaged telomeres.DNA repair · 2018Article
- Endogenous Hot Spots of De Novo Telomere Addition in the Yeast Genome Contain Proximal Enhancers That Bind Cdc13.Molecular and cellular biology · 2016Article
- Crystallographic Studies of Telomerase.Methods in enzymology · 2016Article
- Analysis of epigenetic stability and conversions in Saccharomyces cerevisiae reveals a novel role of CAF-I in position-effect variegation.Nucleic acids research · 2013Article
- Novel phosphorylation sites in the S. cerevisiae Cdc13 protein reveal new targets for telomere length regulation.Journal of proteome research · 2013Article
- Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: beginning to end.Genetics · 2012Review
- Functional diversification of yeast telomere associated protein, Rif1, in higher eukaryotes.BMC genomics · 2012Article
- RPA facilitates telomerase activity at chromosome ends in budding and fission yeasts.The EMBO journal · 2012Article
- Stimulation of gross chromosomal rearrangements by the human CEB1 and CEB25 minisatellites in Saccharomyces cerevisiae depends on G-quadruplexes or Cdc13.PLoS genetics · 2012Article
- An anti-checkpoint activity for rif1.PLoS genetics · 2011Article
- A novel checkpoint and RPA inhibitory pathway regulated by Rif1.PLoS genetics · 2011Article
- The Saccharomyces cerevisiae telomerase subunit Est3 binds telomeres in a cell cycle- and Est1-dependent manner and interacts directly with Est1 in vitro.PLoS genetics · 2011Article
- Cdc13 N-terminal dimerization, DNA binding, and telomere length regulation.Molecular and cellular biology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Although a surprisingly large number of genes affect yeast telomeres, in most cases it is not known if their products act directly or indirectly. We describe a one-hybrid assay for telomere binding proteins and use it to establish that six proteins that affect telomere structure or function but which had not been shown previously to bind telomeres in vivo are indeed telomere binding proteins. A promoter-defective allele of HIS3 was placed adjacent to a chromosomal telomere. Candidate proteins fused to a transcriptional activation domain were tested for the ability to activate transcription of the telomere-linked HIS3 gene. Using this system, Rif1p, Rif2p, Sir2p, Sir3p, Sir4p, and Cdc13p were found to be in vivo telomere binding proteins. None of the proteins activated the same reporter gene when it was at an internal site on the chromosome. Moreover, Cdc13p did not activate the reporter gene when it was adjacent to an internal tract of telomeric sequence, indicating that Cdc13p binding was telomere limited in vivo. The amino-terminal 20% of Cdc13p was sufficient to target Cdc13p to a telomere, suggesting that its DNA binding domain was within this portion of the protein. Rap1p, Rif1p, Rif2p, Sir4p, and Cdc13p activated the telomeric reporter gene in a strain lacking Sir3p, which is essential for telomere position effect (TPE). Thus, the telomeric association of these proteins did not require any of the chromatin features necessary for TPE. The data support models in which the telomere acts as an initiation site for TPE by recruiting silencing proteins to the chromosome end.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.