ArticleMolecular and cellular biology1998
The maternal CCAAT box transcription factor which controls GATA-2 expression is novel and developmentally regulated and contains a double-stranded-RNA-binding subunit.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- The RNA binding complexes NF45-NF90 and NF45-NF110 associate dynamically with the c-fos gene and function as transcriptional coactivators.The Journal of biological chemistry · 2015Article
- RNA granule assembly and disassembly modulated by nuclear factor associated with double-stranded RNA 2 and nuclear factor 45.The Journal of biological chemistry · 2014Article
- Notch signaling represses p63 expression in the developing surface ectoderm.Development (Cambridge, England) · 2013Article
- NF90 in posttranscriptional gene regulation and microRNA biogenesis.International journal of molecular sciences · 2013Review
- Beta-Actin Is Involved in Modulating Erythropoiesis during Development by Fine-Tuning Gata2 Expression Levels.PloS one · 2013Article
- NF45 dimerizes with NF90, Zfr and SPNR via a conserved domain that has a nucleotidyltransferase fold.Nucleic acids research · 2012Article
- ILF-3 is a regulator of the neural plate border marker Zic1 in chick embryos.Developmental dynamics : an official publication of the American Association of Anatomists · 2012Article
- Phosphorylation of the NFAR proteins by the dsRNA-dependent protein kinase PKR constitutes a novel mechanism of translational regulation and cellular defense.Genes & development · 2010Article
- A multiprotein complex necessary for both transcription and DNA replication at the β-globin locus.The EMBO journal · 2010Article
- Smicl is required for phosphorylation of RNA polymerase II and affects 3'-end processing of RNA at the midblastula transition in Xenopus.Development (Cambridge, England) · 2009Article
- NFAR-1 and -2 modulate translation and are required for efficient host defense.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- RNA binding and intramolecular interactions modulate the regulation of gene expression by nuclear factor 110.RNA (New York, N.Y.) · 2003Article
- Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins.The Journal of cell biology · 2002Article
- The RNA binding protein nuclear factor 90 functions as both a positive and negative regulator of gene expression in mammalian cells.Molecular and cellular biology · 2002Article
- RNA-dependent cytoplasmic anchoring of a transcription factor subunit during Xenopus development.The EMBO journal · 2000Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The transcription factor GATA-2 is expressed at high levels in the nonneural ectoderm of the Xenopus embryo at neurula stages, with lower amounts of RNA present in the ventral mesoderm and endoderm. The promoter of the GATA-2 gene contains an inverted CCAAT box conserved among Xenopus laevis, humans, chickens, and mice. We have shown that this sequence is essential for GATA-2 transcription during early development and that the factor binding it is maternal. The DNA-binding activity of this factor is detectable in nuclei and chromatin bound only when zygotic GATA-2 transcription starts. Here we report the characterization of this factor, which we call CBTF (CCAAT box transcription factor). CBTF activity mainly appears late in oogenesis, when it is nuclear, and the complex has multiple subunits. We have identified one subunit of the factor as p122, a Xenopus double-stranded-RNA-binding protein. The p122 protein is perinuclear during early embryonic development but moves from the cytoplasm into the nuclei of embryonic cells at stage 9, prior to the detection of CBTF activity in the nucleus. Thus, the accumulation of CBTF activity in the nucleus is a multistep process. We show that the p122 protein is expressed mainly in the ectoderm. Expression of p122 mRNA is more restricted, mainly to the anterior ectoderm and mesoderm and to the neural tube. Two properties of CBTF, its dual role and its cytoplasm-to-nucleus translocation, are shared with other vertebrate maternal transcription factors and may be general properties of these proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.