ArticleMolecular and cellular biology1998
Requirement for a functional interaction between mediator components Med6 and Srb4 in RNA polymerase II transcription.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- The Mediator Complex: From Transcriptional Regulation to Disease Pathogenesis.International journal of molecular sciences · 2026Review
- Article
- Proteomic Stratification of Prognosis and Treatment Options for Small Cell Lung Cancer.Genomics, proteomics & bioinformatics · 2024Article
- Role of integrative structural biology in understanding transcriptional initiation.Methods (San Diego, Calif.) · 2019Review
- Twenty years of Mediator complex structural studies.Biochemical Society transactions · 2019Review
- Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex.Biochemistry research international · 2018Article
- Article
- Disordered proteinaceous machines.Chemical reviews · 2014Review
- Baculovirus expression: tackling the complexity challenge.Current opinion in structural biology · 2013Review
- A DEAD box RNA helicase is critical for pre-mRNA splicing, cold-responsive gene regulation, and cold tolerance in Arabidopsis.The Plant cell · 2013Article
- Structure of the Mediator head module.Nature · 2012Article
- Facilitated assembly of the preinitiation complex by separated tail and head/middle modules of the mediator.Journal of molecular biology · 2012Article
- Med8, Med18, and Med20 subunits of the Mediator head domain are interdependent upon each other for folding and complex formation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Patterns of co-expression for protein complexes by size in Saccharomyces cerevisiae.Nucleic acids research · 2009Article
- Two conserved modules of Schizosaccharomyces pombe Mediator regulate distinct cellular pathways.Nucleic acids research · 2008Article
- Functional conservation of the glutamine-rich domains of yeast Gal11 and human SRC-1 in the transactivation of glucocorticoid receptor Tau 1 in Saccharomyces cerevisiae.Molecular and cellular biology · 2008Article
- Transcriptional regulators of seven yeast species: comparative genome analysis. Review.Folia microbiologica · 2008Review
- Article
- Basal transcription machinery: role in regulation of stress response in eukaryotes.Journal of biosciences · 2007Review
- Independent recruitment of mediator and SAGA by the activator Met4.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
2 authors.
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Abstract
Regulated transcription of class II genes of the yeast Saccharomyces cerevisiae requires the diverse functions of mediator complex. In particular, MED6 is essential for activated transcription from many class II promoters, suggesting that it functions as a key player in the relay of activator signals to the basal transcription machinery. To identify the functional relationship between MED6 and other transcriptional regulators, we conducted a genetic screen to isolate a suppressor of a temperature-sensitive (ts) med6 mutation. We identified an SRB4 allele as a dominant and allele-specific suppressor of med6-ts. A single missense mutation in SRB4 can specifically suppress transcriptional defects caused by the med6 ts mutation, indicating a functional interaction between these two mediator subunits in the activation of transcription. Biochemical analysis of mediator subassembly revealed that mediator can be dissociated into two tightly associated subcomplexes. The Med6 and Srb4 proteins are contained in the same subcomplex together with other dominant Srb proteins, consistent with their functional relationship revealed by the genetic study. Our results suggest not only the existence of a specific interaction between Med6 and Srb4 but also the requirement of this interaction in transcriptional regulation of RNA polymerase II holoenzyme.
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