ArticleProceedings of the National Academy of Sciences of the United States of America1998
ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of Golgi structure and function.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 94 citations in OpenAlex.
- Cytohesin-2 is essential for the perinatal development of mice and regulates Golgi volume.Life science alliance · 2026Article
- Physiological and Pathological Roles of the Cytohesin Family in Neurons.International journal of molecular sciences · 2022Review
- The C-terminal domain of EFA6A interacts directly with F-actin and assembles F-actin bundles.Scientific reports · 2019Article
- ArfGAP1 restrictsEMBO reports · 2018Article
- The Focal-Focal Preconditioning Effect of Photothrombotic Impact on the Signaling Protein Profile in the Penumbra Surrounding the Ischemic Core Induced by Another Photothrombotic Impact.Molecular neurobiology · 2018Article
- Arf6 exchange factor EFA6 and endophilin directly interact at the plasma membrane to control clathrin-mediated endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Solution structure of subunit a, a₁₀₄₋₃₆₃, of the Saccharomyces cerevisiae V-ATPase and the importance of its C-terminus in structure formation.Journal of bioenergetics and biomembranes · 2012Article
- Kinetic studies of the Arf activator Arno on model membranes in the presence of Arf effectors suggest control by a positive feedback loop.The Journal of biological chemistry · 2011Article
- Variation in genes required for normal mitosis and risk of breast cancer.Breast cancer research and treatment · 2010Article
- ARAP1 regulates endocytosis of EGFR.Traffic (Copenhagen, Denmark) · 2008Article
- Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1.The FEBS journal · 2007Article
- Active Arf6 recruits ARNO/cytohesin GEFs to the PM by binding their PH domains.Molecular biology of the cell · 2007Article
- Phylogenetic analysis of Sec7-domain-containing Arf nucleotide exchangers.Molecular biology of the cell · 2004Article
- Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23.The EMBO journal · 2001Article
- A novel interaction of the Golgi complex with the vimentin intermediate filament cytoskeleton.The Journal of cell biology · 2001Article
- The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- A conserved domain of the arabidopsis GNOM protein mediates subunit interaction and cyclophilin 5 binding.The Plant cell · 2000Article
- p200 ARF-GEP1: a Golgi-localized guanine nucleotide exchange protein whose Sec7 domain is targeted by the drug brefeldin A.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Structural basis for the inhibitory effect of brefeldin A on guanine nucleotide-exchange proteins for ADP-ribosylation factors.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Budding of transport vesicles in the Golgi apparatus requires the recruitment of coat proteins and is regulated by ADP ribosylation factor (ARF) 1. ARF1 activation is promoted by guanine nucleotide exchange factors (GEFs), which catalyze the transition to GTP-bound ARF1. We recently have identified a human protein, ARNO (ARF nucleotide-binding-site opener), as an ARF1-GEF that shares a conserved domain with the yeast Sec7 protein. We now describe a human Sec7 domain-containing GEF referred to as ARNO3. ARNO and ARNO3, as well as a third GEF called cytohesin-1, form a family of highly related proteins with identical structural organization that consists of a central Sec7 domain and a carboxy-terminal pleckstrin homology domain. We show that all three proteins act as ARF1 GEF in vitro, whereas they have no effect on ARF6, an ARF protein implicated in the early endocytic pathway. Substrate specificity of ARNO-like GEFs for ARF1 depends solely on the Sec7 domain. Overexpression of ARNO3 in mammalian cells results in (i) fragmentation of the Golgi apparatus, (ii) redistribution of Golgi resident proteins as well as the coat component beta-COP, and (iii) inhibition of SEAP transport (secreted form of alkaline phosphatase). In contrast, the distribution of endocytic markers is not affected. This study indicates that Sec7 domain-containing GEFs control intracellular membrane compartment structure and function through the regulation of specific ARF proteins in mammalian cells.
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