ArticleThe Plant cell1998
Ca2+, annexins, and GTP modulate exocytosis from maize root cap protoplasts
Article in The Plant cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 156 citations in OpenAlex.
- Annexins: central regulators of plant growth and stress signaling.Acta biochimica et biophysica Sinica · 2025Review
- Extracellular RNA: mechanisms of secretion and potential functions.Journal of experimental botany · 2023Article
- ROS and calcium oscillations are required for polarized root hair growth.Plant signaling & behavior · 2022Review
- A nanodomain-anchored scaffolding complex is required for the function and localization of phosphatidylinositol 4-kinase alpha in plants.The Plant cell · 2022Article
- Calmodulin and Its Interactive Proteins Participate in Regulating the Explosive Growth ofInternational journal of molecular sciences · 2021Article
- The durum wheat annexin, TdAnn6, improves salt and osmotic stress tolerance in Arabidopsis via modulation of antioxidant machinery.Protoplasma · 2021Article
- Salt Tolerance and Na Allocation inPlants (Basel, Switzerland) · 2020Article
- DORN1/P2K1 and purino-calcium signalling in plants: making waves with extracellular ATP.Annals of botany · 2020Article
- Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 inFrontiers in plant science · 2020Article
- Maize annexin genes ZmANN33 and ZmANN35 encode proteins that function in cell membrane recovery during seed germination.Journal of experimental botany · 2019Article
- OsARD4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots.Scientific reports · 2018Article
- Oxidative and genotoxic damages in plants in response to heavy metal stress and maintenance of genome stability.Plant signaling & behavior · 2018Article
- Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy and Its Role in Glaucoma.Frontiers in neurology · 2018Review
- Annexins as Overlooked Regulators of Membrane Trafficking in Plant Cells.International journal of molecular sciences · 2017Review
- Hyphopodium-Specific VdNoxB/VdPls1-Dependent ROS-Ca2+ Signaling Is Required for Plant Infection by Verticillium dahliae.PLoS pathogens · 2016Article
- Comprehensive analyses of the annexin gene family in wheat.BMC genomics · 2016Article
- Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers.Proteomes · 2016Article
- Calcium-Mediated Abiotic Stress Signaling in Roots.Frontiers in plant science · 2016Review
- Comprehensive Tissue-Specific Transcriptome Analysis Reveals Distinct Regulatory Programs during Early Tomato Fruit Development.Plant physiology · 2015Article
- Proteomic analysis of lettuce seed germination and thermoinhibition by sampling of individual seeds at germination and removal of storage proteins by polyethylene glycol fractionation.Plant physiology · 2015Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protoplasts isolated from root cap cells of maize were shown to secrete fucose-rich polysaccharides and were used in a patch-clamp study to monitor changes in whole-cell capacitance. Ca2+ was required for exocytosis, which was measured as an increase in cell capacitance during intracellular dialysis with Ca2+ buffers via the patch pipette. Exocytosis was stimulated significantly by small increases above normal resting [Ca2+]. In the absence of Ca2+, protoplasts decreased in size. In situ hybridization showed significant expression of the maize annexin p35 in root cap cells, differ-entiating vascular tissue, and elongating cells. Dialysis of protoplasts with maize annexins stimulated exocytosis at physiological [Ca2+], and this could be blocked by dialysis with antibodies specific to maize annexins. Dialysis with milli-molar concentrations of GTP strongly inhibited exocytosis, causing protoplasts to decrease in size. GTPgammaS and GDPbetaS both caused only a slight inhibition of exocytosis at physiological Ca2+. Protoplasts were shown to internalize plasma membrane actively. The results are discussed in relation to the regulation of exocytosis in what is usually considered to be a constitutively secreting system; they provide direct evidence for a role of annexins in exocytosis in plant cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.