ArticleThe Journal of physiology1998
Calcineurin involvement in the regulation of high-threshold Ca2+ channels in NG108-15 (rodent neuroblastoma x glioma hybrid) cells.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 34 citations in OpenAlex.
- Localized calcineurin confers Ca2+-dependent inactivation on neuronal L-type Ca2+ channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Modulation of Ca²⁺ signals by epigallocatechin-3-gallate(EGCG) in cultured rat hippocampal neurons.International journal of molecular sciences · 2011Article
- Modulation of calcium-dependent inactivation of L-type Ca2+ channels via β-adrenergic signaling in thalamocortical relay neurons.PloS one · 2011Article
- Targeting of protein phosphatases PP2A and PP2B to the C-terminus of the L-type calcium channel Ca v1.2.Biochemistry · 2010Article
- Hippocampal 'zipper' slice studies reveal a necessary role for calcineurin in the increased activity of L-type Ca(2+) channels with aging.Neurobiology of aging · 2010Article
- Neuroprotection by nicotine in mouse primary cortical cultures involves activation of calcineurin and L-type calcium channel inactivation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Calcineurin plays different roles in group II metabotropic glutamate receptor- and NMDA receptor-dependent long-term depression.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Calcineurin enhances L-type Ca(2+) channel activity in hippocampal neurons: increased effect with age in culture.Neuroscience · 2002Article
- Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- D2 dopamine receptors in striatal medium spiny neurons reduce L-type Ca2+ currents and excitability via a novel PLC[beta]1-IP3-calcineurin-signaling cascade.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Functional IP3- and ryanodine-sensitive calcium stores in presynaptic varicosities of NG108-15 (rodent neuroblastoma x glioma hybrid) cells.The Journal of physiology · 2000Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
1. We examined the relationship between calcineurin (protein phosphatase 2B (PP2B) and voltage-operated Ca2+ channels (VOCCs) in NG108-15 cells. PP2B expression in NG108-15 cells was altered by transfection with plasmid constructs containing a full length cDNA of human PP2B beta(3) in sense (CN-15) and antisense (CN-21) orientation. 2. Confocal immunocytochemical localization showed that in wild-type cells, PP2B immunoreactivity is uniformly distributed in undifferentiated cells and located at the inner surface of soma membrane and neurites in differentiated cells. 3. To test the Ca2+ dependence of the VOCC, we used high-frequency stimulation (HFS). The L- and N-type VOCCs decreased by 37 and 52%, respectively, whereas the T-type current was only marginally sensitive to this procedure. FK-506 (2 microM), a specific blocker of PP2B, reduced the inhibition of L- and N-type VOCCs induced by HFS by 30 and 33%, respectively. 4. In CN-15-transfected cells overexpressing PP2B, total high-voltage-activated (HVA) VOCCs were suppressed by about 60% at a test potential of +20 mV. Intracellular addition of EGTA or FK-506 into CN-15-transfected cells induced an up to 5-fold increase of HVA VOCCs. 5. These findings indicate that PP2B activity does not influence the expression of HVA Ca2+ channels, but modulates their function by Ca(2+)-dependent dephosphorylation. Thus HVA VOCCs, in a phosphorylated state under control conditions, are downregulated by PP2B upon stimulation, with the major effect on N-type VOCCs.
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