ArticleNucleic acids research1998
The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility.iScience · 2024Article
- Loss of FOCAD, operating via the SKI messenger RNA surveillance pathway, causes a pediatric syndrome with liver cirrhosis.Nature genetics · 2022Article
- Mammalian RNA Decay Pathways Are Highly Specialized and Widely Linked to Translation.Molecular cell · 2020Article
- Review
- Noncoding RNA Surveillance: The Ends Justify the Means.Chemical reviews · 2018Review
- DExD/H-box RNA helicases in ribosome biogenesis.RNA biology · 2013Review
- Cardiovirus 2A protein associates with 40S but not 80S ribosome subunits during infection.Journal of virology · 2007Article
- Small protein B interacts with the large and the small subunits of a stalled ribosome during trans-translation.Nucleic acids research · 2006Article
- Decay of mRNAs targeted by RISC requires XRN1, the Ski complex, and the exosome.RNA (New York, N.Y.) · 2005Article
- Linking the 3' poly(A) tail to the subunit joining step of translation initiation: relations of Pab1p, eukaryotic translation initiation factor 5b (Fun12p), and Ski2p-Slh1p.Molecular and cellular biology · 2001Article
- 3' poly(A) is dispensable for translation.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p.Molecular and cellular biology · 2000Article
- A novel helicase-type protein in the nucleolus: protein NOH61.Molecular biology of the cell · 2000Article
- The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo.RNA (New York, N.Y.) · 2000Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The human helicase gene SKI2W is located between RD and RP1 in the class III region of the major histocompatibility complex. Transcripts of SKI2W are detectable in RNA samples isolated from multiple tissues. The protein product Ski2w shares striking amino acid sequence similarities to the yeast antiviral protein Ski2p that controls the translation of mRNAs, probably based on the mRNA structural integrity. Whether this translational regulation mechanism for cellular and viral RNAs exists in mammals is under investigation. Antisera against human Ski2w were generated using fusion proteins produced in bacteria or insect cells. Western blot analysis showed that the endogenous Ski2w protein is approximately 140 kDa in size and is enriched in polysomal fractions of cytoplasmic extracts from HeLa cells. Ribosomal profile studies revealed that Ski2w distributed throughout the entire sucrose gradient in the presence of Mg2+, but co-sedimented with the 18S rRNA-containing 40S subunit and the small ribosomal subunit protein S27a in the presence of EDTA. The co-sedimentation of Ski2w with the 40S subunit is not affected by RNase A treatment of the cell extract, or the addition of KCl to 0.5 M, suggesting that Ski2w is associated with the 40S ribosomal subunit. Indirect immunofluorescence experiments showed that human Ski2w is localized in the nucleoli and in the cytoplasm. In essence, human Ski2w is present at the sites of ribosome biogenesis and protein synthesis.
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Registered trials
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