ArticleNucleic acids research1998
Mutations in the yeast Myb-like protein Bas1p resulting in discrimination between promoters in vivo but notin vitro.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 24 citations in OpenAlex.
- Identification of yeast and human 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAr) transporters.The Journal of biological chemistry · 2014Article
- Regulation of amino acid, nucleotide, and phosphate metabolism in Saccharomyces cerevisiae.Genetics · 2012Article
- Physiological and toxic effects of purine intermediate 5-amino-4-imidazolecarboxamide ribonucleotide (AICAR) in yeast.The Journal of biological chemistry · 2011Article
- Article
- Metabolic intermediates selectively stimulate transcription factor interaction and modulate phosphate and purine pathways.Genes & development · 2009Article
- Max-E47, a designed minimalist protein that targets the E-box DNA site in vivo and in vitro.Journal of the American Chemical Society · 2009Article
- DNA-bound Bas1 recruits Pho2 to activate ADE genes in Saccharomyces cerevisiae.Eukaryotic cell · 2005Article
- Severe adenine starvation activates Ty1 transcription and retrotransposition in Saccharomyces cerevisiae.Molecular and cellular biology · 2005Article
- Guanylic nucleotide starvation affects Saccharomyces cerevisiae mother-daughter separation and may be a signal for entry into quiescence.BMC cell biology · 2005Article
- Yeast AMP pathway genes respond to adenine through regulated synthesis of a metabolic intermediate.Molecular and cellular biology · 2001Article
- A novel yeast system for in vivo selection of recognition sequences: defining an optimal c-Myb-responsive element.Nucleic acids research · 2001Article
- Highly conserved features of DNA binding between two divergent members of the Myb family of transcription factors.Nucleic acids research · 2001Article
- Signaling through regulated transcription factor interaction: mapping of a regulatory interaction domain in the Myb-related Bas1p.Nucleic acids research · 2000Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Bas1p is a yeast transcription factor that activates expression of purine and histidine biosynthesis genes in response to extracellular purine limitation. The N-terminal part of Bas1p contains an Myb-like DNA binding domain composed of three tryptophan-rich imperfect repeats. We show that mutating the conserved tryptophan residues in the DNA binding domain of Bas1p severely impairs in vivo activation of target genes and in vitro DNA binding of Bas1p. We also found that two mutations (H34L and W42A) in the first repeat make Bas1p discriminate between promoters in vivo . These two BAS1 mutants are able to activate expression of an HIS4-lacZ fusion but not that of ADE1-lacZ or ADE17-lacZ fusions. Surprisingly, these mutant proteins bind equally well to the three promoters in vitro , suggesting that the mutations affect the interaction of Bas1p with some promoter-specific factor(s) in vivo . By mutating a potential nucleotide binding site in the DNA binding domain of Bas1p, we also show that this motif does not play a major role in purine regulation of Bas1p activity. Finally, using a green fluorescence protein (GFP)-Bas1p fusion, we establish the strict nuclear localization of Bas1p and show that it is not affected by extracellular adenine.
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