Evidence map›Paper›PMID 9705505›Full record

ArticleNucleic acids research1998

Cloning, characterization and expression of the gene coding for a cytosine-5-DNA methyltransferase recognizing GpC.

M Xu, M P Kladde, J L Van Etten, R T Simpson

Open access · bronzeAbstract readComparative Study
In one paragraph

Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 85 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Phage-encoded ten-eleven translocation dioxygenase (TET) is active in C5-cytosine hypermodification in DNA.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  16. Chromatin accessibility profiling methods.Nature reviews. Methods primers · 2021
    Article
  17. Chloroviruses.Viruses · 2019
    Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

M XuDepartment of Biochemistry and Molecular Biology and the Center for Gene Regulation, 308 Althouse Laboratory,The Pennsylvania State University, University Park, PA 16802-4500, USA.
M P Kladde
J L Van Etten
R T Simpson
Pennsylvania State University · US

Funding

GENOME AND GENETICS OF A EUKARYOTIC ALGAL VIRUSR01GM032441 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI VAN ETTEN, JAMES L · 1985 to 2009
$2.8M
CHROMATIN STRUCTURE IN LIVING CELLSR01GM052908 · NIGMS · PENNSYLVANIA STATE UNIVERSITY-UNIV PARK · PI SCHLEGEL, ROBERT A · 1995 to 2003
$1.1M
NIGMS NIH HHS GM32441NIGMS NIH HHS GM52908NIGMS NIH HHS R01 GM052908
6 · The paper itself

Abstract

A novel gene encoding a cytosine-5-DNA methyltransferase recognizing the dinucleotide GpC was cloned from Chlorella virus NYs-1 and expressed in both Escherichia coli and Saccharomyces cerevisiae . The gene was sequenced and a predicted polypeptide of 362 amino acids with a molecular weight of 41.903 kDa was identified. The protein contains several amino acid motifs with high similarity to those of other known 5-methylcytosine-forming methyltransferases. In addition, this enzyme, named M. Cvi PI, shares 66% identity and 76% similarity with M. Cvi JI, the only other cytosine-5-DNA methyltransferase cloned from a Chlorella virus. The short, frequently occurring recognition sequence of the new methyltransferase will be very useful for in vivo chromatin structure studies in both yeast and higher organisms.

Indexed as

Amino Acid SequenceChlorellaChromatinChromosome MappingCloning, MolecularDinucleoside PhosphatesDNA (Cytosine-5-)-MethyltransferasesDNA PrimersEscherichia coliGenes, ViralGenomic LibraryMethylationMolecular Sequence DataPhycodnaviridaeSaccharomyces cerevisiaeSequence Homology, Amino Acid2'-deoxyguanylyl-(3'-5')-2'-deoxycytidineChromatinDinucleoside PhosphatesDNA (Cytosine-5-)-MethyltransferasesDNA Primers

Identifiers

PMID9705505
PMCPMC147793
OpenAlexW2116805270

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.