ArticleJournal of clinical microbiology1998
Development of rRNA-targeted PCR and in situ hybridization with fluorescently labelled oligonucleotides for detection of Yersinia species.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 117 citations in OpenAlex.
- Applications of polymerase chain reaction-based methods for the diagnosis of plague (Review).Experimental and therapeutic medicine · 2022Review
- Exposure to bacterial and fungal bioaerosols in facilities processing biodegradable waste.Frontiers in public health · 2022Article
- Obtaining Specific Sequence Tags forPathogens (Basel, Switzerland) · 2021Article
- NOTIFy (non-toxic lyophilized field)-FISH for the identification of biological agents by Fluorescence in situ Hybridization.PloS one · 2020Article
- Detection of 16S rRNA and KPC Genes from Complex Matrix Utilizing a Molecular Inversion Probe Assay for Next-Generation Sequencing.Scientific reports · 2018Article
- Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial.Journal of clinical microbiology · 2015Article
- Homology analysis of pathogenic Yersinia species Yersinia enterocolitica, Yersinia pseudotuberculosis, and Yersinia pestis based on multilocus sequence typing.Journal of clinical microbiology · 2014Article
- Article
- Ecological significance of microdiversity: coexistence among casing soil bacterial strains through allocation of nutritional resource.Indian journal of microbiology · 2011Article
- Rapid and sensitive detection of Yersinia pestis using amplification of plague diagnostic bacteriophages monitored by real-time PCR.PloS one · 2010Article
- Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA.BMC microbiology · 2010Article
- TaqMan-based real-time PCR method for detection of Yersinia pseudotuberculosis in food.Applied and environmental microbiology · 2008Article
- Current trends in plague research: from genomics to virulence.Clinical medicine & research · 2006Review
- Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.Applied and environmental microbiology · 2006Article
- Serotype differences and lack of biofilm formation characterize Yersinia pseudotuberculosis infection of the Xenopsylla cheopis flea vector of Yersinia pestis.Journal of bacteriology · 2006Article
- A hypervariable N-terminal region of Yersinia LcrV determines Toll-like receptor 2-mediated IL-10 induction and mouse virulence.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Spatial organization and composition of the mucosal flora in patients with inflammatory bowel disease.Journal of clinical microbiology · 2005Article
- Multilocus sequence typing for studying genetic relationships among Yersinia species.Journal of clinical microbiology · 2005Article
- Spatial organization of bacterial flora in normal and inflamed intestine: a fluorescence in situ hybridization study in mice.World journal of gastroenterology · 2005Article
- Ecological significance of microdiversity: identical 16S rRNA gene sequences can be found in bacteria with highly divergent genomes and ecophysiologies.Applied and environmental microbiology · 2004Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this report, we present details of two rapid molecular detection techniques based on 16S and 23S rRNA sequence data to identify and differentiate Yersinia species from clinical and environmental sources. Near-full-length 16S rRNA gene (rDNA) sequences for three different Yersinia species and partial 23S rDNA sequences for three Y. pestis and three Y. pseudotuberculosis strains were determined. While 16S rDNA sequences of Y. pestis and Y. pseudotuberculosis were found to be identical, one base difference was identified within a highly variable region of 23S rDNA. The rDNA sequences were used to develop primers and fluorescently tagged oligonucleotide probes suitable for differential detection of Yersinia species by PCR and in situ hybridization, respectively. As few as 10(2) Yersinia cells per ml could be detected by PCR with a seminested approach. Amplification with a subgenus-specific primer pair followed by a second PCR allowed differentiation of Y. enterocolitica biogroup 1B from biogroups 2 to 5 or from other pathogenic Yersinia species. Moreover, a set of oligonucleotide probes suitable for rapid (3-h) in situ detection and differentiation of the three pathogenic Yersinia species (in particular Y. pestis and Y. pseudotuberculosis) was developed. The applicability of this technique was demonstrated by detection of Y. pestis and Y. pseudotuberculosis in spiked throat and stool samples, respectively. These probes were also capable of identifying Y. enterocolitica within cryosections of experimentally infected mouse tissue by the use of confocal laser scanning microscopy.
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