ArticleJournal of clinical microbiology1998
Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Rapid Diagnostic ofMicroorganisms · 2023Article
- Prospective Analysis of Antibody Diagnostic Tests and TTS1 Real-Time PCR for Diagnosis of Melioidosis in Areas Where It Is Endemic.Journal of clinical microbiology · 2023Article
- Biodistribution and immunity of adenovirus 5/35 and modified vaccinia Ankara vector vaccines against human immunodeficiency virus 1 clade C.Gene therapy · 2022Article
- Rapid detection ofPeerJ · 2021Article
- Canine Brucellosis: An Update.Frontiers in veterinary science · 2021Review
- Diagnosis of canine brucellosis: comparison of various serologic tests and PCR.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Detection and quantification of Erysipelothrix rhusiopathiae in blood from infected chickens - addressing challenges with detection of DNA from infectious agents in host species with nucleated red blood cells.Journal of medical microbiology · 2019Article
- Detection ofMicromachines · 2019Article
- Emerging Technologies for Molecular Diagnosis of Sepsis.Clinical microbiology reviews · 2018Review
- Direct DNA and RNA detection from large volumes of whole human blood.Scientific reports · 2018Article
- A prospective multicenter evaluation of direct molecular detection of blood stream infection from a clinical perspective.BMC infectious diseases · 2016Observational
- Rapid Point-of-Care Isothermal Amplification Assay for the Detection of Malaria without Nucleic Acid Purification.Infectious diseases · 2016Article
- Improved sensitivity for molecular detection of bacterial and Candida infections in blood.Journal of clinical microbiology · 2014Article
- Polymerase chain reaction-based assays for the diagnosis of human brucellosis.Annals of clinical microbiology and antimicrobials · 2014Review
- DNA sequencing diagnosis of off-season spirochetemia with low bacterial density in Borrelia burgdorferi and Borrelia miyamotoi infections.International journal of molecular sciences · 2014Article
- Fluorescence resonance energy transfer-based real-time polymerase chain reaction method without DNA extraction for the genotyping of F5, F2, F12, MTHFR, and HFE.Journal of blood medicine · 2014Article
- Quality control methods for optimal BCR-ABL1 clinical testing in human whole blood samples.The Journal of molecular diagnostics : JMD · 2013Article
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Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by increasing the number of washings to four or five and decreasing the amount of total DNA to 2 to 4 microg, thereby avoiding false-negative results.
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