ArticleJournal of clinical microbiology1998
Microsatellite markers for typing Aspergillus fumigatus isolates.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Voriconazole for chronic pulmonary aspergillosis: a prospective multicenter trial.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Trial
- Short Tandem Repeat Genotyping of Medically Important Fungi: A Comprehensive Review of a Powerful Tool with Extensive Future Potential.Mycopathologia · 2024Review
- Azole resistance mechanisms and population structure of the human pathogenApplied and environmental microbiology · 2024Article
- Review
- Molecular Epidemiology ofJournal of fungi (Basel, Switzerland) · 2021Review
- Comparison of Two Highly Discriminatory Typing Methods to AnalyzeFrontiers in microbiology · 2018Article
- Isolation of azole-resistant Aspergillus fumigatus from the environment in the south-eastern USA.The Journal of antimicrobial chemotherapy · 2017Article
- Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?Clinical microbiology reviews · 2017Review
- Article
- Article
- Highly discriminatory variable-number tandem-repeat markers for genotyping of Trichophyton interdigitale strains.Journal of clinical microbiology · 2014Article
- Microsatellite typing of Aspergillus flavus from clinical and environmental avian isolates.Journal of medical microbiology · 2013Article
- Azole preexposure affects the Aspergillus fumigatus population in patients.Antimicrobial agents and chemotherapy · 2012Article
- Interrogation of related clinical pan-azole-resistant Aspergillus fumigatus strains: G138C, Y431C, and G434C single nucleotide polymorphisms in cyp51A, upregulation of cyp51A, and integration and activation of transposon Atf1 in the cyp51A promoter.Antimicrobial agents and chemotherapy · 2011Article
- Molecular epidemiology of Aspergillus fumigatus: an in-depth genotypic analysis of isolates involved in an outbreak of invasive aspergillosis.Journal of clinical microbiology · 2011Article
- Azole resistance in Aspergillus fumigatus isolates from the ARTEMIS global surveillance study is primarily due to the TR/L98H mutation in the cyp51A gene.Antimicrobial agents and chemotherapy · 2011Article
- A review molecular typing methods for Aspergillus flavus isolates.Mycopathologia · 2011Review
- High resolution genotyping of clinical Aspergillus flavus isolates from India using microsatellites.PloS one · 2011Article
- Aspergillus fumigatus in Poultry.International journal of microbiology · 2011Article
- Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus.BMC microbiology · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of microsatellites as highly polymorphic DNA markers for the typing of isolates of Aspergillus fumigatus was investigated. Four CA repeats were selected by screening an A. fumigatus DNA library with a (CA)10 oligonucleotide. Primers flanking these CA repeats were designed to amplify each locus. One primer of each pair was labeled with a fluorophore, and the PCR products were analyzed with an automatic sequencer and the GeneScan software. For each primer set and for a given isolate, one band was detected and was assigned to an allele because A. fumigatus is haploid. With 50 clinical isolates, 50 environmental isolates, and 2 reference strains we obtained 12, 11, 10, and 23 different alleles for the four CA microsatellites, respectively (discriminatory power, 0.994). The results were identical by whatever DNA extraction technique was used. Interestingly, no clustering between environmental and clinical isolates was observed, suggesting that every isolate is potentially pathogenic. Microsatellite markers appear suitable for use in large epidemiological studies of invasive aspergillosis.
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