ArticlePlant physiology1998
Intracellular beta-carbonic anhydrase of the unicellular green alga Coccomyxa. Cloning of the cdna and characterization of the functional enzyme overexpressed in Escherichia coli.
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 49 citations in OpenAlex.
- Expression of seven carbonic anhydrases in red alga Gracilariopsis chorda and their subcellular localization in a heterologous system, Arabidopsis thaliana.Plant cell reports · 2019Article
- Redox regulation of carbonic anhydrases via thioredoxin in chloroplast of the marine diatom Phaeodactylum tricornutum.The Journal of biological chemistry · 2012Article
- Structural studies of β-carbonic anhydrase from the green alga Coccomyxa: inhibitor complexes with anions and acetazolamide.PloS one · 2011Article
- Cloning, polymorphism, and inhibition of beta-carbonic anhydrase of Helicobacter pylori.Journal of gastroenterology · 2008Article
- Characterization of the carboxysomal carbonic anhydrase CsoSCA from Halothiobacillus neapolitanus.Journal of bacteriology · 2006Article
- Historical perspective on microalgal and cyanobacterial acclimation to low- and extremely high-CO(2) conditions.Photosynthesis research · 2003Article
- Physiological and molecular biological characterization of intracellular carbonic anhydrase from the marine diatom Phaeodactylum tricornutum.Plant physiology · 2001Article
- Inhibition of photosynthesis by intracellular carbonic anhydrase in microalgae under excess concentrations of CO(2).Photosynthesis research · 2001Article
- The active site architecture of Pisum sativum beta-carbonic anhydrase is a mirror image of that of alpha-carbonic anhydrases.The EMBO journal · 2000Article
- A plant-type (beta-class) carbonic anhydrase in the thermophilic methanoarchaeon Methanobacterium thermoautotrophicum.Journal of bacteriology · 1999Article
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5 authors at 1 institution in 1 country.
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Abstract
Carbonic anhydrase (CA) (EC 4.2.1.1) enzymes catalyze the reversible hydration of CO2, a reaction that is important in many physiological processes. We have cloned and sequenced a full-length cDNA encoding an intracellular beta-CA from the unicellular green alga Coccomyxa. Nucleotide sequence data show that the isolated cDNA contains an open reading frame encoding a polypeptide of 227 amino acids. The predicted polypeptide is similar to beta-type CAs from Escherichia coli and higher plants, with an identity of 26% to 30%. The Coccomyxa cDNA was overexpressed in E. coli, and the enzyme was purified and biochemically characterized. The mature protein is a homotetramer with an estimated molecular mass of 100 kD. The CO2-hydration activity of the Coccomyxa enzyme is comparable with that of the pea homolog. However, the activity of Coccomyxa CA is largely insensitive to oxidative conditions, in contrast to similar enzymes from most higher plants. Fractionation studies further showed that Coccomyxa CA is extrachloroplastic.
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