ArticleThe Journal of cell biology1998
Interactions of the cytoplasmic domain of P-selectin with clathrin-coated pits enhance leukocyte adhesion under flow.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- P-selectin mobility undergoes a sol-gel transition as it diffuses from exocytosis sites into the cell membrane.Nature communications · 2022Article
- Thromboinflammation Model-on-A-Chip by Whole Blood Microfluidics on Fixed Human Endothelium.Diagnostics (Basel, Switzerland) · 2021Article
- Role of NO and S-nitrosylation in the Expression of Endothelial Adhesion Proteins That Regulate Leukocyte and Tumor Cell Adhesion.Frontiers in physiology · 2020Review
- Endothelial signaling by neutrophil-released oncostatin M enhances P-selectin-dependent inflammation and thrombosis.Blood advances · 2019Article
- The Interaction of Selectins and PSGL-1 as a Key Component in Thrombus Formation and Cancer Progression.BioMed research international · 2017Review
- Adhesion molecules in CNS disorders: biomarker and therapeutic targets.CNS & neurological disorders drug targets · 2013Review
- Article
- A new approach to explore the binding space of polysaccharide-based ligands: selectin antagonists.ACS medicinal chemistry letters · 2012Article
- The essential role of clathrin-mediated endocytosis in the infectious entry of human enterovirus 71.The Journal of biological chemistry · 2011Article
- Biomechanics of leukocyte rolling.Biorheology · 2011Review
- Rolling cell adhesion.Annual review of cell and developmental biology · 2010Review
- Polymorphonuclear leukocytes isolated from umbilical cord blood as a useful research tool to study adherence to cell monolayers.Journal of immunological methods · 2009Article
- Separable requirements for cytoplasmic domain of PSGL-1 in leukocyte rolling and signaling under flow.Blood · 2008Article
- Article
- Remodeling of the lectin-EGF-like domain interface in P- and L-selectin increases adhesiveness and shear resistance under hydrodynamic force.Nature immunology · 2006Article
- Endocytic trafficking routes of wild type and DeltaF508 cystic fibrosis transmembrane conductance regulator.Molecular biology of the cell · 2004Article
- Signal-dependent distribution of cell surface P-selectin in clathrin-coated pits affects leukocyte rolling under flow.The Journal of cell biology · 2003Article
- Anti-adhesive effect of nitric oxide on Plasmodium falciparum cytoadherence under flow.The American journal of pathology · 2003Article
- Distinct molecular and cellular contributions to stabilizing selectin-mediated rolling under flow.The Journal of cell biology · 2002Article
- Cytoplasmic anchorage of L-selectin controls leukocyte capture and rolling by increasing the mechanical stability of the selectin tether.The Journal of cell biology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Flowing leukocytes tether to and roll on P-selectin, a receptor on endothelial cells that is rapidly internalized in clathrin-coated pits. We asked whether the association of P-selectin with clathrin-coated pits contributes to its adhesive function. Under flow, rolling neutrophils accumulated efficiently on CHO cells expressing wild-type P-selectin or a P-selectin construct with a substitution in the cytoplasmic domain that caused even faster internalization than that of the wild-type protein. By contrast, far fewer rolling neutrophils accumulated on CHO cells expressing P-selectin constructs with a deletion or a substitution in the cytoplasmic domain that impaired internalization. Neutrophils rolled on the internalization-competent constructs with greater adhesive strength, slower velocity, and more uniform motion. Flowing neutrophils tethered equivalently to internalization-competent or internalization-defective P-selectin, but after tethering, they rolled further on internalization-competent P-selectin. Confocal microscopy demonstrated colocalization of alpha-adaptin, a component of clathrin-coated pits, with wild-type P-selectin, but not with P-selectin lacking the cytoplasmic domain. Treatment of CHO cells or endothelial cells with hypertonic medium reversibly impaired the clathrin-mediated internalization of P-selectin and its ability to support neutrophil rolling. Interactions of the cytoplasmic domain of P-selectin with clathrin-coated pits provide a novel mechanism to enhance leukocyte adhesion under flow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.