ArticleThe Journal of cell biology1998
Recruitment of the gamma-tubulin ring complex to Drosophila salt-stripped centrosome scaffolds.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 232 citations in OpenAlex.
- The γ-Tubulin Ring Complex promotes mitotic spindle integrity and acts as a microtubule minus-end cap during mitosis.bioRxiv : the preprint server for biology · 2026Article
- The conserved Spd-2/CEP192 domain adopts a unique protein fold to promote centrosome scaffold assembly.Science advances · 2025Article
- Versatile gamma-tubulin complexes contribute to the dynamic organization of MTOCs during Drosophila spermatogenesis.Communications biology · 2024Article
- Amplified centrosomes-more than just a threat.EMBO reports · 2024Review
- Structure of the γ-tubulin ring complex-capped microtubule.Nature structural & molecular biology · 2024Article
- Microtubule nucleation for spindle assembly: one molecule at a time.Trends in biochemical sciences · 2023Review
- A germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans.Journal of cell science · 2023Article
- The ring saga: looking back at the discovery of γ-tubulin and γ-tubulin ring complexes.Molecular biology of the cell · 2023Article
- Article
- The centriolar satellite protein Cfap53 facilitates formation of the zygotic microtubule organizing center in the zebrafish embryo.Development (Cambridge, England) · 2022Article
- Molecular insight into how γ-TuRC makes microtubules.Journal of cell science · 2021Review
- Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly.Journal of cell science · 2021Article
- The γ-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells.Communications biology · 2021Article
- Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome.The Journal of cell biology · 2021Article
- Centriole-independent mitotic spindle assembly relies on the PCNT-CDK5RAP2 pericentriolar matrix.The Journal of cell biology · 2020Article
- Non-Canonical Functions of the Gamma-Tubulin Meshwork in the Regulation of the Nuclear Architecture.Cancers · 2020Review
- Review
- XMAP215 and γ-tubulin additively promote microtubule nucleation in purified solutions.Molecular biology of the cell · 2020Article
- Article
- Choreography of the centrosome.Heliyon · 2020Review
39 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Extracting isolated Drosophila centrosomes with 2 M KI generates salt-resistant scaffolds that lack the centrosomal proteins CP190, CP60, centrosomin, and gamma-tubulin. To clarify the role of these proteins in microtubule nucleation by centrosomes and to identify additional centrosome components required for nucleation, we have developed an in vitro complementation assay for centrosome function. Centrosome aster formation is reconstituted when these inactive, salt-stripped centrosome scaffolds are supplemented with a soluble fraction of a Drosophila embryo extract. The CP60 and CP190 can be removed from this extract without effect, whereas removing the gamma-tubulin destroys the complementing activity. Consistent with these results, we find no evidence that these three proteins form a complex together. Instead, gamma-tubulin is found in two distinct protein complexes of 240,000 and approximately 3,000,000 D. The larger complex, which is analogous to the Xenopus gamma-tubulin ring complex (gammaTuRC) (Zheng, Y., M.L. Wong, B. Alberts, and T. Mitchison. 1995. Nature. 378:578-583), is necessary but not sufficient for complementation. An additional factor found in the extract is required. These results provide the first evidence that the gammaTuRC is required for microtubule nucleation at the centrosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.