ArticleThe Journal of cell biology1998
Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 111 citations in OpenAlex.
- Regulation of Yeast Cytokinesis by Calcium.Journal of fungi (Basel, Switzerland) · 2025Review
- Loss of Septation Initiation Network (SIN) kinases blocks tissue invasion and unlocks echinocandin cidal activity against Aspergillus fumigatus.PLoS pathogens · 2021Article
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- Comparing the utility of in vivo transposon mutagenesis approaches in yeast species to infer gene essentiality.Current genetics · 2020Article
- Yeast Rgd3 is a phospho-regulated F-BAR-containing RhoGAP involved in the regulation of Rho3 distribution and cell morphology.Molecular biology of the cell · 2020Article
- Comparative Analysis of the Roles of Non-muscle Myosin-IIs in Cytokinesis in Budding Yeast, Fission Yeast, and Mammalian Cells.Frontiers in cell and developmental biology · 2020Review
- Precise Post-translational Tuning Occurs for Most Protein Complex Components during Meiosis.Cell reports · 2018Article
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- Origin and evolution of fungal HECT ubiquitin ligases.Scientific reports · 2018Article
- Mechanics and regulation of cytokinesis in budding yeast.Seminars in cell & developmental biology · 2017Review
- Actomyosin ring driven cytokinesis in budding yeast.Seminars in cell & developmental biology · 2016Review
- Fluorescent markers for the Spitzenkörper and exocytosis in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Myosin‑II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis.Molecular biology of the cell · 2015Article
- Targeting and functional mechanisms of the cytokinesis-related F-BAR protein Hof1 during the cell cycle.Molecular biology of the cell · 2013Article
- Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast.The Journal of cell biology · 2013Article
- Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method.Genome research · 2013Article
- Yeast vacuoles fragment in an asymmetrical two-phase process with distinct protein requirements.Molecular biology of the cell · 2012Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
- Yeast homologues of lethal giant larvae and type V myosin cooperate in the regulation of Rab-dependent vesicle clustering and polarized exocytosis.Molecular biology of the cell · 2011Article
- Cooperation between the septins and the actomyosin ring and role of a cell-integrity pathway during cell division in fission yeast.Genetics · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, the unconventional myosin Myo2p is of fundamental importance in polarized growth. We explore the role of the neck region and its associated light chains in regulating Myo2p function. Surprisingly, we find that precise deletion of the six IQ sites in the neck region results in a myosin, Myo2-Delta6IQp, that can support the growth of a yeast strain at 90% the rate of a wild-type isogenic strain. We exploit this mutant in a characterization of the light chains of Myo2p. First, we demonstrate that the localization of calmodulin to sites of polarized growth largely depends on the IQ sites in the neck of Myo2p. Second, we demonstrate that a previously uncharacterized protein, Mlc1p, is a myosin light chain of Myo2p. MLC1 (YGL106w) is an essential gene that exhibits haploinsufficiency. Reduced levels of MYO2 overcome the haploinsufficiency of MLC1. The mutant MYO2-Delta6IQ is able to suppress haploinsufficiency but not deletion of MLC1. We used a modified gel overlay assay to demonstrate a direct interaction between Mlc1p and the neck of Myo2p. Overexpression of MYO2 is toxic, causing a severe decrease in growth rate. When MYO2 is overexpressed, Myo2p is fourfold less stable than in a wild-type strain. High copies of MLC1 completely overcome the growth defects and increase the stability of Myo2p. Our results suggest that Mlc1p is responsible for stabilizing this myosin by binding to the neck region.
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