ArticlePlant molecular biology1998
Functional organization of the cassava vein mosaic virus (CsVMV) promoter.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 100 citations in OpenAlex.
- A study on the influence of different promoter and 5'UTR (URM) cassettes from Arabidopsis thaliana on the expression level of the reporter gene β glucuronidase in tobacco and cotton.Transgenic research · 2014Pooled it
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- Impacts of environmental conditions, and allelic variation of cytosolic glutamine synthetase on maize hybrid kernel production.Communications biology · 2021Article
- Identification and characterization of a new soybean promoter induced by Phakopsora pachyrhizi, the causal agent of Asian soybean rust.BMC biotechnology · 2021Article
- Overexpression ofInternational journal of molecular sciences · 2021Article
- The novel protein CSAP accelerates leaf senescence and is negatively regulated by SAUL1 in the dark.Plant cell reports · 2020Article
- Transcription Terminator-Mediated Enhancement in Transgene Expression in Maize: Preponderance of the AUGAAU Motif Overlapping With Poly(A) Signals.Frontiers in plant science · 2020Article
- Zinc finger nuclease-mediated targeting of multiple transgenes to an endogenous soybean genomic locus via non-homologous end joining.Plant biotechnology journal · 2019Article
- Isolation, characterization, and evaluation of three Citrus sinensis-derived constitutive gene promoters.Plant cell reports · 2018Article
- Agronomic and chemical performance of field-grown tobacco engineered for triterpene and methylated triterpene metabolism.Plant biotechnology journal · 2018Article
- Whitefly and aphid inducible promoters of Arabidopsis thaliana L.Journal of genetics · 2018Article
- Recombinant Promoter (MUASCsV8CP) Driven Totiviral Killer Protein 4 (KP4) Imparts Resistance Against Fungal Pathogens in Transgenic Tobacco.Frontiers in plant science · 2018Article
- Control of Amino Acid Homeostasis by a Ubiquitin Ligase-Coactivator Protein Complex.The Journal of biological chemistry · 2017Article
- Development of a Triple GeneFrontiers in plant science · 2017Article
- Artificial microRNA-derived resistance to Cassava brown streak disease.Journal of virological methods · 2016Article
- A Virus-Derived Stacked RNAi Construct Confers Robust Resistance to Cassava Brown Streak Disease.Frontiers in plant science · 2016Article
- Article
- Isolation and functional characterization of a novel rice constitutive promoter.Plant cell reports · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cassava vein mosaic virus (CsVMV) is a pararetrovirus that infects cassava plants in Brazil. A promoter fragment isolated from CsVMV, comprising nucleotides -443 to +72, was previously shown to direct strong constitutive gene expression in transgenic plants. Here we report the functional architecture of the CsVMV promoter fragment. A series of promoter deletion mutants were fused to the coding sequence of uidA reporter gene and the chimeric genes were introduced into transgenic tobacco plants. Promoter activity was monitored by histochemical and quantitative assays of beta-glucuronidase activity (GUS). We found that the promoter fragment is made up of different regions that confer distinct tissue-specific expression of the gene. The region encompassing nucleotides -222 to -173 contains cis elements that control promoter expression in green tissues and root tips. Our results indicate that a consensus as1 element and a GATA motif located within this region are essential for promoter expression in those tissues. Expression from the CsVMV promoter in vascular elements is directed by the region encompassing nucleotides -178 to -63. Elements located between nucleotides -149 and -63 are also required to activate promoter expression in green tissues suggesting a combinatorial mode of regulation. Within the latter region, a 43 bp fragment extending from nucleotide -141 to -99 was shown to interact with a protein factor extracted from nuclei of tobacco seedlings. This fragment showed no sequence homology with other pararetrovirus promoters and hence may contain CsVMV-specific regulatory cis elements.
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