ArticleJournal of virology1998
Rotavirus infection reduces sucrase-isomaltase expression in human intestinal epithelial cells by perturbing protein targeting and organization of microvillar cytoskeleton.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 113 citations in OpenAlex.
- Little Patients Big Discoveries: Potential Pediatric to Adult Neurogastroenterology Translation.The American journal of gastroenterology · 2025Review
- The Role of the Host Cytoskeleton in the Formation and Dynamics of Rotavirus Viroplasms.Viruses · 2024Review
- Prevalence of Worldwide Neonatal Calf Diarrhoea Caused by Bovine Rotavirus in Combination with Bovine Coronavirus,Animals : an open access journal from MDPI · 2021Review
- Article
- A modified lysosomal organelle mediates nonlytic egress of reovirus.The Journal of cell biology · 2020Article
- Congenital Lactase Deficiency: Mutations, Functional and Biochemical Implications, and Future Perspectives.Nutrients · 2019Review
- Rotavirus-Induced Early Activation of the RhoA/ROCK/MLC Signaling Pathway Mediates the Disruption of Tight Junctions in Polarized MDCK Cells.Scientific reports · 2018Article
- Actin-Dependent Nonlytic Rotavirus Exit and Infectious Virus Morphogenetic Pathway in Nonpolarized Cells.Journal of virology · 2018Article
- Article
- Molecular Survey of Viral and Bacterial Causes of Childhood Diarrhea in Khartoum State, Sudan.Frontiers in microbiology · 2018Article
- Glucose enhances rotavirus enterotoxin-induced intestinal chloride secretion.Pflugers Archiv : European journal of physiology · 2017Article
- Structure-function analysis of human sucrase-isomaltase identifies key residues required for catalytic activity.The Journal of biological chemistry · 2017Article
- Rice Bran and Probiotics Alter the Porcine Large Intestine and Serum Metabolomes for Protection against Human Rotavirus Diarrhea.Frontiers in microbiology · 2017Article
- The multiple roles of sucrase-isomaltase in the intestinal physiology.Molecular and cellular pediatrics · 2016Article
- Pathogen-induced secretory diarrhea and its prevention.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Review
- Diversity in Rotavirus-Host Glycan Interactions: A "Sweet" Spectrum.Cellular and molecular gastroenterology and hepatology · 2016Review
- Activation of calcium/calmodulin-dependent kinase II following bovine rotavirus enterotoxin NSP4 expression.Iranian journal of basic medical sciences · 2015Article
- Comparative proteomic analysis reveals activation of mucosal innate immune signaling pathways during cholera.Infection and immunity · 2015Article
- [Epidemiological and clinical study of rotavirus acute diarrhea in infants at the hospital Jason Sendwe Lubumbashi, Democratic Republic of Congo].The Pan African medical journal · 2015Article
- Characteristic and functional analysis of a newly established porcine small intestinal epithelial cell line.PloS one · 2014Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rotavirus infection is the most common cause of severe infantile gastroenteritis worldwide. These viruses infect mature enterocytes of the small intestine and cause structural and functional damage, including a reduction in disaccharidase activity. It was previously hypothesized that reduced disaccharidase activity resulted from the destruction of rotavirus-infected enterocytes at the villus tips. However, this pathophysiological model cannot explain situations in which low disaccharidase activity is observed when rotavirus-infected intestine exhibits few, if any, histopathologic changes. In a previous study, we demonstrated that the simian rotavirus strain RRV replicated in and was released from human enterocyte-like Caco-2 cells without cell destruction (N. Jourdan, M. Maurice, D. Delautier, A. M. Quero, A. L. Servin, and G. Trugnan, J. Virol. 71:8268-8278, 1997). In the present study, to reinvestigate disaccharidase expression during rotavirus infection, we studied sucrase-isomaltase (SI) in RRV-infected Caco-2 cells. We showed that SI activity and apical expression were specifically and selectively decreased by RRV infection without apparent cell destruction. Using pulse-chase experiments and cell surface biotinylation, we demonstrated that RRV infection did not affect SI biosynthesis, maturation, or stability but induced the blockade of SI transport to the brush border. Using confocal laser scanning microscopy, we showed that RRV infection induces important alterations of the cytoskeleton that correlate with decreased SI apical surface expression. These results lead us to propose an alternate model to explain the pathophysiology associated with rotavirus infection.
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