ArticleJournal of bacteriology1998
Insertion mutation of the form I cbbL gene encoding ribulose bisphosphate carboxylase/oxygenase (RuBisCO) in Thiobacillus neapolitanus results in expression of form II RuBisCO, loss of carboxysomes, and an increased CO2 requirement for growth.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Synergistic Inorganic Carbon and Denitrification Genes Contributed to Nitrite Accumulation in a Hydrogen-Based Membrane Biofilm Reactor.Bioengineering (Basel, Switzerland) · 2022Article
- Decoding the Absolute Stoichiometric Composition and Structural Plasticity of α-Carboxysomes.mBio · 2022Article
- Exploring the oxygenase function of Form II Rubisco for production of glycolate from COAMB Express · 2021Article
- Functional reconstitution of a bacterial COeLife · 2020Article
- Engineering and Modulating Functional Cyanobacterial COFrontiers in plant science · 2018Article
- Direct characterization of the native structure and mechanics of cyanobacterial carboxysomes.Nanoscale · 2017Article
- Structural Characterization of a Newly Identified Component of α-Carboxysomes: The AAA+ Domain Protein CsoCbbQ.Scientific reports · 2015Article
- Advances in Understanding Carboxysome Assembly in Prochlorococcus and Synechococcus Implicate CsoS2 as a Critical Component.Life (Basel, Switzerland) · 2015Article
- Functions, compositions, and evolution of the two types of carboxysomes: polyhedral microcompartments that facilitate CO2 fixation in cyanobacteria and some proteobacteria.Microbiology and molecular biology reviews : MMBR · 2013Review
- Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells.Journal of molecular biology · 2010Article
- The pentameric vertex proteins are necessary for the icosahedral carboxysome shell to function as a CO2 leakage barrier.PloS one · 2009Article
- Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species.PloS one · 2008Article
- Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome.PLoS biology · 2007Article
- Development of a genetic system for the chemolithoautotrophic bacterium Thiobacillus denitrificans.Applied and environmental microbiology · 2007Article
- CO2-responsive expression and gene organization of three ribulose-1,5-bisphosphate carboxylase/oxygenase enzymes and carboxysomes in Hydrogenovibrio marinus strain MH-110.Journal of bacteriology · 2004Article
- A novel evolutionary lineage of carbonic anhydrase (epsilon class) is a component of the carboxysome shell.Journal of bacteriology · 2004Article
- Microcompartments in prokaryotes: carboxysomes and related polyhedra.Applied and environmental microbiology · 2001Review
- The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins.Journal of bacteriology · 1999Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been previously established that Thiobacillus neapolitanus fixes CO2 by using a form I ribulose bisphosphate carboxylase/oxygenase (RuBisCO), that much of the enzyme is sequestered into carboxysomes, and that the genes for the enzyme, cbbL and cbbS, are part of a putative carboxysome operon. In the present study, cbbL and cbbS were cloned and sequenced. Analysis of RNA showed that cbbL and cbbS are cotranscribed on a message approximately 2,000 nucleotides in size. The insertion of a kanamycin resistance cartridge into cbbL resulted in a premature termination of transcription; a polar mutant was generated. The mutant is able to fix CO2, but requires a CO2 supplement for growth. Separation of cellular proteins from both the wild type and the mutant on sucrose gradients and subsequent analysis of the RuBisCO activity in the collected fractions showed that the mutant assimilates CO2 by using a form II RuBisCO. This was confirmed by immunoblot analysis using antibodies raised against form I and form II RuBisCOs. The mutant does not possess carboxysomes. Smaller, empty inclusions are present, but biochemical analysis indicates that if they are carboxysome related, they are not functional, i.e., do not contain RuBisCO. Northern analysis showed that some of the shell components of the carboxysome are produced, which may explain the presence of these inclusions in the mutant.
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