ArticleGenes & development1998
Enhancer control of V(D)J recombination at the TCRbeta locus: differential effects on DNA cleavage and joining.
Article in Genes & development, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 70 citations in OpenAlex.
- Novel bimodal TRBD1-TRBD2 rearrangements with dual or absent D-region contribute to TRB V-(D)-J combinatorial diversity.Frontiers in immunology · 2023Article
- Characterization of human αβTCR repertoire and discovery of D-D fusion in TCRβ chains.Protein & cell · 2014Article
- V(D)J recombination: Born to be wild.Seminars in cancer biology · 2010Review
- Leaky severe combined immunodeficiency and aberrant DNA rearrangements due to a hypomorphic RAG1 mutation.Blood · 2009Article
- RAG2's non-core domain contributes to the ordered regulation of V(D)J recombination.Nucleic acids research · 2008Article
- Genomic organization and recombinational unit duplication-driven evolution of ovine and bovine T cell receptor gamma loci.BMC genomics · 2008Article
- In vivo reinsertion of excised episomes by the V(D)J recombinase: a potential threat to genomic stability.PLoS biology · 2007Article
- Reassignment of the murine 3'TRDD1 recombination signal sequence.Immunogenetics · 2006Article
- Activation of p38 MAP kinase by DNA double-strand breaks in V(D)J recombination induces a G2/M cell cycle checkpoint.The EMBO journal · 2006Article
- The T cell receptor beta enhancer promotes access and pairing of Dbeta and Jbeta gene segments during V(D)J recombination.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Article
- Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Restrictions limiting the generation of DNA double strand breaks during chromosomal V(D)J recombination.The Journal of experimental medicine · 2002Article
- Regulation of antigen receptor gene assembly in lymphocytes.Immunologic research · 2001Review
- Chromatin remodeling by the T cell receptor (TCR)-beta gene enhancer during early T cell development: Implications for the control of TCR-beta locus recombination.The Journal of experimental medicine · 2000Article
- TCRalpha enhancer activation occurs via a conformational change of a pre-assembled nucleo-protein complex.The EMBO journal · 2000Article
- The interleukin 7 receptor is required for T cell receptor gamma locus accessibility to the V(D)J recombinase.The Journal of experimental medicine · 2000Article
- Definition of a T-cell receptor beta gene core enhancer of V(D)J recombination by transgenic mapping.Molecular and cellular biology · 2000Article
- Regulation of V(D)J recombination by transcriptional promoters.Molecular and cellular biology · 1999Article
- Analysis of variable (diversity) joining recombination in DNAdependent protein kinase (DNA-PK)-deficient mice reveals DNA-PK-independent pathways for both signal and coding joint formation.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Deletion of the TCRbeta transcriptional enhancer (Ebeta) results in nearly complete inhibition of V(D)J recombination at the TCRbeta locus and a block in alpha beta T cell development. This result, along with previous work from many laboratories, has led to the hypothesis that transcriptional enhancers affect V(D)J recombination by regulating the accessibility of the locus to the recombinase. Here we test this hypothesis by performing a detailed analysis of the recombination defect in Ebeta-deleted (Ebeta-/-) mice using assays that detect various reaction intermediates and products. We found double-strand DNA breaks at recombination signal sequences flanking Dbeta and Jbeta gene segments in Ebeta-/- thymuses at about one-third to one-thirtieth the level found in thymuses with an unaltered TCRbeta locus. These sites are also subject to in vitro cleavage by the V(D)J recombinase in both Ebeta-/- and Ebeta+/+ thymocyte nuclei. However, the corresponding Dbeta-to-Jbeta coding joints are further reduced (by 100- to 300-fold) in Ebeta-/- thymuses. Formation of extrachromosomal Dbeta-to-Jbeta signal joints appears to be intermediately affected and nonstandard Dbeta-to-Dbeta joining occurs at the Ebeta-deleted alleles. These data indicate that, unexpectedly, loss of accessibility alone cannot explain the loss of TCRbeta recombination in the absence of the Ebeta element and suggest an additional function for Ebeta in the process of DNA repair at specific TCRbeta sites during the late phase of the recombination reaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.