ArticleMolecular biology of the cell1998
ATP- and cytosol-dependent release of adaptor proteins from clathrin-coated vesicles: A dual role for Hsc70.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 90 citations in OpenAlex.
- How much (ATP) does it cost to build a trypanosome? A theoretical study on the quantity of ATP needed to maintain and duplicate a bloodstream-form Trypanosoma brucei cell.PLoS pathogens · 2023Article
- Control of clathrin-mediated endocytosis by NIMA family kinases.PLoS genetics · 2020Article
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- Article
- A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating.Cell discovery · 2018Article
- New links between vesicle coats and Rab-mediated vesicle targeting.Seminars in cell & developmental biology · 2011Review
- Transport vesicle uncoating: it's later than you think.F1000 biology reports · 2010Article
- Coordinated regulation of AP2 uncoating from clathrin-coated vesicles by rab5 and hRME-6.The Journal of cell biology · 2008Article
- A novel AAK1 splice variant functions at multiple steps of the endocytic pathway.Molecular biology of the cell · 2007Article
- The J-domain protein Rme-8 interacts with Hsc70 to control clathrin-dependent endocytosis in Drosophila.The Journal of cell biology · 2004Article
- AP-1 binding to sorting signals and release from clathrin-coated vesicles is regulated by phosphorylation.The Journal of cell biology · 2003Article
- Adaptor and clathrin exchange at the plasma membrane and trans-Golgi network.Molecular biology of the cell · 2003Article
- Hsc70 is required for endocytosis and clathrin function in Drosophila.The Journal of cell biology · 2002Article
- Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis.The Journal of cell biology · 2002Article
- Hsc/Hsp70 interacting protein (hip) associates with CXCR2 and regulates the receptor signaling and trafficking.The Journal of biological chemistry · 2002Article
- Human stoned B interacts with AP-2 and synaptotagmin and facilitates clathrin-coated vesicle uncoating.EMBO reports · 2001Article
- Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo.The Journal of cell biology · 2001Article
- Vesicular protein transport.The pharmacogenomics journal · 2001Review
- Membrane-associated GAIP is a phosphoprotein and can be phosphorylated by clathrin-coated vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Wrapping the package.Proceedings of the National Academy of Sciences of the United States of America · 1999Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Clathrin-coated vesicles (CCV) mediate protein sorting and vesicular trafficking from the plasma membrane and the trans-Golgi network. Before delivery of the vesicle contents to the target organelles, the coat components, clathrin and adaptor protein complexes (APs), must be released. Previous work has established that hsc70/the uncoating ATPase mediates clathrin release in vitro without the release of APs. AP release has not been reconstituted in vitro, and nothing is known about the requirements for this reaction. We report a novel quantitative assay for the ATP- and cytosol- dependent release of APs from CCV. As expected, hsc70 is not sufficient for AP release; however, immunodepletion and reconstitution experiments establish that it is necessary. Interestingly, complete clathrin release is not a prerequisite for AP release, suggesting that hsc70 plays a dual role in recycling the constituents of the clathrin coat. This assay provides a functional basis for identification of the additional cytosolic factor(s) required for AP release.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.