ArticleMolecular biology of the cell1998
Efficient trafficking of TGN38 from the endosome to the trans-Golgi network requires a free hydroxyl group at position 331 in the cytosolic domain.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 52 citations in OpenAlex.
- PIKI-1, a class II PI 3-kinase, functions in endocytic trafficking.PLoS genetics · 2026Article
- PIKI-1, a class II phosphatidylinositol 3-kinase, functions in endocytic trafficking.bioRxiv : the preprint server for biology · 2025Article
- Cargo selective vesicle tethering: The structural basis for binding of specific cargo proteins by the Golgi tether component TBC1D23.Science advances · 2024Article
- Functional characterization of endocytic signals in the SynDIG/PRRT family members SynDIG1 and SynDIG4 in heterologous cells and neurons.Frontiers in cellular neuroscience · 2024Article
- Conserved NIMA kinases regulate multiple steps of endocytic trafficking.PLoS genetics · 2023Article
- Structural and functional studies of TBC1D23 C-terminal domain provide a link between endosomal trafficking and PCH.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Radical Stress Is More Cytotoxic in the Nucleus than in Other Organelles.International journal of molecular sciences · 2019Article
- A recurrent missense variant in SLC9A7 causes nonsyndromic X-linked intellectual disability with alteration of Golgi acidification and aberrant glycosylation.Human molecular genetics · 2019Article
- Dynamic changes in copper homeostasis and post-transcriptional regulation of Atp7a during myogenic differentiation.Metallomics : integrated biometal science · 2018Article
- TMEM30A is a candidate interacting partner for the β-carboxyl-terminal fragment of amyloid-β precursor protein in endosomes.PloS one · 2018Article
- TBC1D23 is a bridging factor for endosomal vesicle capture by golgins at the trans-Golgi.Nature cell biology · 2017Article
- Cargo-selective SNX-BAR proteins mediate retromer trimer independent retrograde transport.The Journal of cell biology · 2017Article
- SORCS1 is necessary for normal insulin secretory granule biogenesis in metabolically stressed β cells.The Journal of clinical investigation · 2014Article
- Rab9-dependent retrograde transport and endosomal sorting of the endopeptidase furin.Journal of cell science · 2011Article
- The open reading frame 3a protein of severe acute respiratory syndrome-associated coronavirus promotes membrane rearrangement and cell death.Journal of virology · 2010Article
- Roles for the recycling endosome, Rab8, and Rab11 in hantavirus release from epithelial cells.Virology · 2008Article
- Increasing the expression of calcium-permeable TRPC3 and TRPC7 channels enhances constitutive secretion.The Biochemical journal · 2008Article
- Trafficking of varicella-zoster virus glycoprotein gI: T(338)-dependent retention in the trans-Golgi network, secretion, and mannose 6-phosphate-inhibitable uptake of the ectodomain.Journal of virology · 2000Article
- Effective formation of major histocompatibility complex class II-peptide complexes from endogenous antigen by thyroid epithelial cells.Immunology · 2000Article
- Specificity of interaction between adaptor-complex medium chains and the tyrosine-based sorting motifs of TGN38 and lgp120.The Biochemical journal · 1998Article
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2 authors at 1 institution in 1 country.
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Abstract
TGN38 is one of the few known resident integral membrane proteins of the trans-Golgi network (TGN). Since it cycles constitutively between the TGN and the plasma membrane, TGN38 is ideally suited as a model protein for the identification of post-Golgi trafficking motifs. Several studies, employing chimeric constructs to detect such motifs within the cytosolic domain of TGN38, have identified the sequence 333YQRL336 as an autonomous signal capable of localizing reporter proteins to the TGN. In addition, one group has found that an upstream serine residue, S331, may also play a role in TGN38 localization. However, the nature and degree of participation of S331 in the localization of TGN38 remain uncertain, and the effect has been studied in chimeric constructs only. Here we investigate the role of S331 in the context of full-length TGN38. Mutations that abolish the hydroxyl moiety at position 331 (A, D, and E) lead to missorting of endocytosed TGN38 to the lysosome. Conversely, mutation of S331 to T has little effect on the endocytic trafficking of TGN38. Together, these findings indicate that the S331 hydroxyl group has a direct or indirect effect on the ability of the cytosolic tail of TGN38 to interact with trafficking and/or sorting machinery at the level of the early endosome. In addition, mutation of S331 to either A or D results in increased levels of TGN38 at the cell surface. The results confirm that S331 plays a critical role in the intracellular trafficking of TGN38 and further reveal that TGN38 undergoes a signal-mediated trafficking step at the level of the endosome.
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