ArticleMolecular biology of the cell1998
Mutational effect of fission yeast polalpha on cell cycle events.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- DNA Polymerase α has pyrimidine dimer translesion activity that is suppressed during normal replication.bioRxiv : the preprint server for biology · 2026Article
- Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.Nucleic acids research · 2019Article
- Regulation of the Cell Cycle and Inflammatory Arthritis by the Transcription CofactorJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- Role of swi7H4 mutant allele of DNA polymerase α in the DNA damage checkpoint response.PloS one · 2015Article
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- Reducing DNA polymerase alpha in the absence of Drosophila ATR leads to P53-dependent apoptosis and developmental defects.Genetics · 2007Article
- Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint.Genes & development · 2005Article
- Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress.Genes & development · 2005Article
- Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27.BMC molecular biology · 2004Article
- A coordinated temporal interplay of nucleosome reorganization factor, sister chromatin cohesion factor, and DNA polymerase alpha facilitates DNA replication.Molecular and cellular biology · 2004Article
- Germinating fission yeast spores delay in G1 in response to UV irradiation.BMC cell biology · 2004Article
- The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.Molecular and cellular biology · 2004Article
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- Replication proteins influence the maintenance of telomere length and telomerase protein stability.Molecular and cellular biology · 2003Article
- Checkpoint activation regulates mutagenic translesion synthesis.Genes & development · 2003Article
- Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.Genetics · 2001Article
- Fission yeast Rad17 associates with chromatin in response to aberrant genomic structures.Molecular and cellular biology · 2001Article
- Regulation of initiation of S phase, replication checkpoint signaling, and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast.Molecular biology of the cell · 2001Article
- Role of fission yeast primase catalytic subunit in the replication checkpoint.Molecular biology of the cell · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Polalpha is the principal DNA polymerase for initiation of DNA replication and also functions in postinitiation DNA synthesis. In this study, we investigated the cell cycle responses induced by mutations in polalpha+. Germinating spores carrying either a deletion of polalpha+ (polalphaDelta) or a structurally intact but catalytically dead polalpha mutation proceed to inappropriate mitosis with no DNA synthesis. This suggests that the catalytic function, and not the physical presence of Polalpha, is required to generate the signal that prevents the cells from entering mitosis prematurely. Cells with a polalphats allele arrest the cell cycle near the hydroxyurea arrest point, but, surprisingly, polalphats in cdc20 (polepsilon mutant) background arrested with a cdc phenoytpe, not a polalphats-like phenotype. At 25 degrees C, replication perturbation caused by polalphats alleles induces Cds1 kinase activity and requires the checkpoint Rads, Cds1, and Rqh1, but not Chk1, to maintain cell viability. At 36 degrees C, replication disruption caused by polalphats alleles induces the phosphorylation of Chk1; however, mutant cells arrest with heterogeneous cell sizes with a population of the cells entering aberrant mitosis. Together, our results indicate that the initiation DNA structure synthesized by Polalpha is required to bring about the S phase to mitosis checkpoint, whereas replication defects of different severity caused by polalphats mutations induce differential downstream kinase responses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.