ArticleGenetics1998
Efficient repair of all types of single-base mismatches in recombination intermediates in Chinese hamster ovary cells. Competition between long-patch and G-T glycosylase-mediated repair of G-T mismatches.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 58 citations in OpenAlex.
- The GC-content at the 5' ends of human protein-coding genes is undergoing mutational decay.Genome biology · 2024Article
- Predicting prime editing efficiency and product purity by deep learning.Nature biotechnology · 2023Article
- Synergistic binding of actinomycin D and echinomycin to DNA mismatch sites and their combined anti-tumour effects.Nucleic acids research · 2023Article
- Strand asymmetry influences mismatch resolution during a single-strand annealing.Genome biology · 2022Article
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- Cytosine Methylation Affects the Mutability of Neighboring Nucleotides in Germline and Soma.Genetics · 2020Article
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- GC-biased gene conversion in yeast is specifically associated with crossovers: molecular mechanisms and evolutionary significance.Molecular biology and evolution · 2013Article
- The sex-specific impact of meiotic recombination on nucleotide composition.Genome biology and evolution · 2012Article
- A genome-wide view of mutation rate co-variation using multivariate analyses.Genome biology · 2011Article
- Substitution patterns are under different influences in primates and rodents.Genome biology and evolution · 2011Article
- Mechanisms of recombination between diverged sequences in wild-type and BLM-deficient mouse and human cells.Molecular and cellular biology · 2010Article
- Identifying concerted evolution and gene conversion in mammalian gene pairs lasting over 100 million years.BMC evolutionary biology · 2009Article
- The correlation between recombination rate and dinucleotide bias in Drosophila melanogaster.Journal of molecular evolution · 2008Article
- The impact of recombination on nucleotide substitutions in the human genome.PLoS genetics · 2008Article
- Article
- GC content evolution of the human and mouse genomes: insights from the study of processed pseudogenes in regions of different recombination rates.Journal of molecular evolution · 2006Article
- Weak selection and recent mutational changes influence polymorphic synonymous mutations in humans.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Measurement of DNA mismatch repair activity in live cells.Nucleic acids research · 2004Article
- Suppression of intrachromosomal gene conversion in mammalian cells by small degrees of sequence divergence.Genetics · 1999Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Repair of all 12 single-base mismatches in recombination intermediates was investigated in Chinese hamster ovary cells. Extrachromosomal recombination was stimulated by double-strand breaks in regions of shared homology. Recombination was predicted to occur via single-strand annealing, yielding heteroduplex DNA (hDNA) with a single mismatch. Nicks were expected on opposite strands flanking hDNA, equidistant from the mismatch. Unlike studies of covalently closed artificial hDNA substrates, all mismatches were efficiently repaired, consistent with a nick-driven repair process. The average repair efficiency for all mispairs was 92%, with no significant differences among mispairs. There was significant strand-independent repair of G-T --> G-C, with a slightly greater bias in a CpG context. Repair of C-A was also biased (toward C-G), but no A-C --> G-C bias was found, a possible sequence context effect. No other mismatches showed evidence of biased repair, but among hetero-mismatches, the trend was toward retention of C or G vs. A or T. Repair of both T-T and G-T mismatches was much less efficient in mismatch repair-deficient cells (approximately 25%), and the residual G-T repair was completely biased toward G-C. Our data indicate that single-base mismatches in recombination intermediates are substrates for at least two competing repair systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.