ArticleProceedings of the National Academy of Sciences of the United States of America1998
Transgenes are dispensable for the RNA degradation step of cosuppression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 118 citations in OpenAlex.
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- The plant siRNA landscape.The Plant cell · 2024Review
- Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology.Frontiers in plant science · 2021Review
- Genetically Modified Organism-Free RNA Interference: Exogenous Application of RNA Molecules in Plants.Plant physiology · 2020Article
- Delivery of Hairpin RNAs and Small RNAs Into Woody and Herbaceous Plants by Trunk Injection and Petiole Absorption.Frontiers in plant science · 2018Article
- Efficient Generation of diRNAs Requires Components in the Posttranscriptional Gene Silencing Pathway.Scientific reports · 2017Article
- A 22-nt artificial microRNA mediates widespread RNA silencing in Arabidopsis.The Plant journal : for cell and molecular biology · 2013Article
- Scion on a stock producing siRNAs of potato spindle tuber viroid (PSTVd) attenuates accumulation of the viroid.PloS one · 2013Article
- Article
- Stable expression and phenotypic impact of attacin E transgene in orchard grown apple trees over a 12 year period.BMC biotechnology · 2010Article
- Developmentally early and late onset of Rps10 silencing in Arabidopsis thaliana: genetic and environmental regulation.Journal of experimental botany · 2009Article
- Tobacco mosaic virus movement protein enhances the spread of RNA silencing.PLoS pathogens · 2008Article
- A multidirectional non-cell autonomous control and a genetic interaction restricting tobacco etch virus susceptibility in Arabidopsis.PloS one · 2007Article
- An SNF2 protein associated with nuclear RNA silencing and the spread of a silencing signal between cells in Arabidopsis.The Plant cell · 2007Article
- Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus.Plant molecular biology · 2007Article
- Transport of sequence-specific RNA interference information between cells.Annual review of genetics · 2007Review
- Aureusvirus P14 is an efficient RNA silencing suppressor that binds double-stranded RNAs without size specificity.Journal of virology · 2005Article
- Grafting the way to the systemic silencing signal in plants.PLoS biology · 2004Article
- Long-term stability of transgene expression driven by barley endosperm-specific hordein promoters in transgenic barley.Plant cell reports · 2003Article
- Functional characterization of the nuclear localization signal for a suppressor of posttranscriptional gene silencing.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cosuppression results in the degradation of RNA from host genes and homologous transgenes after transcription in the nucleus. By using grafting experiments, we have shown previously that a systemic signal mediates the propagation of cosuppression of Nia host genes and 35S-Nia2 transgenes from silenced 35S-Nia2 transgenic stocks to nonsilenced 35S-Nia2 transgenic scions but not to wild-type scions. Here, we examined the requirements for triggering and maintenance of cosuppression in various types of scions. Grafting-induced silencing occurred in 35S-Nia2 transgenic lines over-accumulating Nia mRNA whether they are able to spontaneously trigger cosuppression or not and in 35S-Nia2 transgene-free plants over-accumulating host Nia mRNA caused by metabolic derepression. When grafting-induced silenced scions were removed from the silenced stocks and regrafted onto wild-type plants, silencing was not maintained in the 35S-Nia2 transgene-free plants and in the 35S-Nia2 transgenic lines that are not able to trigger cosuppression spontaneously. Conversely, silencing was maintained in the 35S-Nia2 transgenic lines that are able to trigger cosuppression spontaneously. Our results indicate that the presence of a 35S-Nia2 transgene is dispensable for the RNA degradation step of posttranscriptional silencing when host Nia mRNA over-accumulate above the level of wild-type plants. They also suggest that grafting-induced RNA degradation does not result in the production of the systemic silencing signal required for spontaneous triggering and maintenance.
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