ArticleProceedings of the National Academy of Sciences of the United States of America1998
The disassembly and reassembly of functional centrosomes in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
68 citing papers in PubMed, 183 citations in OpenAlex.
- Mesoscale regulation of microtubule-organizing centers by the E3 ligase TRIM37.Nature structural & molecular biology · 2025Article
- Isolation and manipulation of meiotic spindles from mouse oocytes reveals migration regulated by pulling force during asymmetric division.bioRxiv : the preprint server for biology · 2025Article
- Molecular architectures of centrosomes in C. elegans embryos visualized by cryo-electron tomography.Developmental cell · 2025Article
- The conserved Spd-2/CEP192 domain adopts a unique protein fold to promote centrosome scaffold assembly.Science advances · 2025Article
- Article
- Catalytic growth in a shared enzyme pool ensures robust control of centrosome size.bioRxiv : the preprint server for biology · 2024Article
- Native molecular architectures of centrosomes inbioRxiv : the preprint server for biology · 2024Article
- The ring saga: looking back at the discovery of γ-tubulin and γ-tubulin ring complexes.Molecular biology of the cell · 2023Article
- The centriolar satellite protein Cfap53 facilitates formation of the zygotic microtubule organizing center in the zebrafish embryo.Development (Cambridge, England) · 2022Article
- Human centrosome organization and function in interphase and mitosis.Seminars in cell & developmental biology · 2021Review
- Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly.Journal of cell science · 2021Article
- Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome.The Journal of cell biology · 2021Article
- Centriole-independent mitotic spindle assembly relies on the PCNT-CDK5RAP2 pericentriolar matrix.The Journal of cell biology · 2020Article
- Phase separation of the Cep63•Cep152 complex underlies the formation of dynamic supramolecular self-assemblies at human centrosomes.Cell cycle (Georgetown, Tex.) · 2020Article
- A self-assembled cylindrical platform for Plk4-induced centriole biogenesis.Open biology · 2020Review
- The Emerging Role of ncRNAs and RNA-Binding Proteins in Mitotic Apparatus Formation.Non-coding RNA · 2020Review
- Article
- Centrosome function and assembly in animal cells.Nature reviews. Molecular cell biology · 2015Review
- Isotropic incorporation of SPD-5 underlies centrosome assembly in C. elegans.Current biology : CB · 2015Article
- Probing mammalian centrosome structure using BioID proximity-dependent biotinylation.Methods in cell biology · 2015Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Animal cells contain a single centrosome that nucleates and organizes a polarized array of microtubules which functions in many cellular processes. In most cells the centrosome is composed of two centrioles surrounded by an ill-defined "cloud" of pericentriolar material. Recently, gamma-tubulin-containing 25-nm diameter ring structures have been identified as likely microtubule nucleation sites within the pericentriolar material of isolated centrosomes. Here we demonstrate that when Spisula centrosomes are extracted with 1.0 M KI they lose their microtubule nucleation potential and appear by three-dimensional electron microscopy as a complex lattice, built from 12- to 15-nm thick elementary fiber(s), that lack centrioles and 25-nm rings. Importantly, when these remnants are incubated in extracts prepared from Spisula oocytes they recover their 25-nm rings, gamma-tubulin, and microtubule nucleation potential. This recovery process occurs in the absence of microtubules, divalent cations, and nucleotides. Thus, in animals the centrosome is structurally organized around a KI-insoluble filament-based "centromatrix" that serves as a scaffold to which those proteins required for microtubule nucleation bind, either directly or indirectly, in a divalent cation and nucleotide independent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.