ArticleProceedings of the National Academy of Sciences of the United States of America1998
Single-particle selection and alignment with heavy atom cluster-antibody conjugates.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
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- Gold nanoparticles and tilt pairs to assess protein flexibility by cryo-electron microscopy.Ultramicroscopy · 2021Article
- Fusion of DARPin to Aldolase Enables Visualization of Small Protein by Cryo-EM.Structure (London, England : 1993) · 2019Article
- While the revolution will not be crystallized, biochemistry reigns supreme.Protein science : a publication of the Protein Society · 2017Review
- High-affinity gold nanoparticle pin to label and localize histidine-tagged protein in macromolecular assemblies.Structure (London, England : 1993) · 2014Article
- Fabs enable single particle cryoEM studies of small proteins.Structure (London, England : 1993) · 2012Article
- Determination of Rigidity of Protein Bound Au(144) Clusters by Electron Cryomicroscopy.The journal of physical chemistry. C, Nanomaterials and interfaces · 2010Article
- Synthesis and bioconjugation of 2 and 3 nm-diameter gold nanoparticles.Bioconjugate chemistry · 2010Article
- Gold nanoparticle-protein arrays improve resolution for cryo-electron microscopy.Journal of structural biology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A method is proposed for selecting and aligning images of single biological particles to obtain high-resolution structural information by cryoelectron microscopy. The particles will be labeled with multiple heavy atom clusters to permit the precise determination of particle locations and relative orientations even when imaged close to focus with a low electron dose, conditions optimal for recording high-resolution detail. Heavy atom clusters should also allow selection of images free from many kinds of defects, including specimen movement and particle inhomogeneity. Heavy atom clusters may be introduced in a general way by the construction of "adaptor" molecules based on single-chain Fv antibody fragments, consisting of a constant framework region engineered for optimal cluster binding and a variable antigen binding region selected for a specific target. The success of the method depends on the mobility of the heavy atom cluster on the particle, on the precision to which clusters can be located in an image, and on the sufficiency of cluster projections alone to orient and select particles for averaging. The necessary computational algorithms were developed and implemented in simulations that address the feasibility of the method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.